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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.
Application References:
1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP) 2. Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC IHC) 3. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.
CD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α/β T cells express high levels of CD5, very few γ/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.
Other Names:
Lyt-1, Ly-1, T1, Tp67, Ly-12
Structure:
member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD
Distribution:
Thymocytes, T cells, B-1 cells
Function:
Negative regulator of T-B cell interaction
Ligand Receptor:
CD72, gp35-37
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kipps TJ. 1988. Adv. Immunol. 47:117. 3. Antin JH, et al. 1985. J. Immunol. 136:505. 4. Tarakhovsky A, et al. 1995. Science 269:535.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD5
C57BL/6 mouse splenocytes stained with biotinylated 53-7.3, followed by Sav-PE
FITC anti-mouse CD5
C57BL/6 mouse splenocytes stained with 53-7.3 FITC
PE anti-mouse CD5
C57BL/6 mouse splenocytes stained with 53-7.3 PE
PE/Cy5 anti-mouse CD5
C57BL/6 mouse splenocytes stained with 53-7.3 PE/CY5
Purified anti-mouse CD5
C57BL/6 mouse splenocytes stained with purified 53-7.3, followed by anti-rat IgGs-FITC
Alexa Fluor® 488 anti-mouse CD5
C57BL/6 mouse splenocytes stained with 53-7.3 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD5
C57BL/6 mouse splenocytes stained with 53-7.3 Alexa Fluor® 647
PerCP anti-mouse CD5
C57BL/6 mouse splenocytes stained with 53-7.3 PerCP
