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PE anti-mouse CD49a Antibody
PE anti-mouse CD49a Antibody
142603 25 µg ¥22,000     
142604 100 µg ¥49,000     
Product Details
Clone: HMα1
Isotype: Armenian Hamster IgG
Isotype Control:PE Armenian Hamster IgG Isotype Ctrl
Reactivity: Mouse
Immunogen: Mouse Neuroblastoma Cell Line C1300
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.
COA:
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Application
Notes:

Additional reported applications (for the relevant format) include: inhibition of cell adhesion and cytokine production1,2.
Application
References:

1. Miyake S, et al. 1994. Eur. J. Immunol. 24:2000. (FC, Block)
2. Tanaka T, et al. 1995. Int. Immunol. 7:1183. (Block)
C1300 (mouse neuroblastoma cell line)
C1300 (mouse neuroblastoma cell line) cells were stained with CD49a (clone HMα1) PE (filled histogram) or Armenian hamster IgG PE isotype control (open histogram).


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See PE spectral data



Description:

CD49a is a 1179 aa, type I transmembrane glycoprotein also known as α1 integrin, VLA-1 α chain, or integrin α1. It associates antibody v042010 with CD29 (β1 integrin) to form the VLA-1 complex, a collagen IV and alminin-1 receptor that is expressed on activated T cells, smooth muscle cells, endothelial cells, neuronal cells, fibroblasts, and mesenchymal cells. CD49a is an adhesion molecule and is involved in the regulation of leukocyte migration, T cell proliferation, and cytokine production.
Other Names: Very late antigen 1 (VLA1), integrin alpha 1 (ITGA1)
Structure: 1179 aa, type I transmembrane glycoprotein
Distribution: Endothelial cells, fibroblasts, activated T cells
Function: Leukocyte adhesion and homing; costimulation
Ligand Receptor: Collagens, laminin
Antigen
References:

1. Barczyk M, et al. 2010. Cell Tissue Res. 339:269.
GeneID: 109700
Latest Publications: View the latest CD49a articles on HighwirePress.com
UniProt: View information about CD49a on UniProt.org
Keywords: PE anti-mouse CD49a, HMα1, PE, Very late antigen 1 (VLA1), integrin alpha 1 (ITGA1), Mouse, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-mouse CD49a
    C1300 (mouse adenocarcinoma cell line)

    C1300 (mouse adenocarcinoma cell line) cells were stained with purified CD49a (clone HMα1, filled histogram) or Armenian hamster IgG (open histogram), followed by anti-hamster IgG PE.

  • PE anti-mouse CD49a
    C1300 (mouse neuroblastoma cell line)

    C1300 (mouse neuroblastoma cell line) cells were stained with CD49a (clone HMα1) PE (filled histogram) or Armenian hamster IgG PE isotype control (open histogram).

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