The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, complement-dependent cytotoxicity1,3, and immunohistochemistry2,5 of acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 103120).
Application References:
1. Ledbetter, J.A., et al., 1979. Immunol. Rev. 47:63. 2. Simon, D.I., et al., 2000. J. Clin. Invest. 105:293. 3. Seaman, W.E., 1983. J. Immunol. 130:1713. 4. Podd, B.S., et al., 2006. J. Immunol. 176: 6532. 5. Cornet, A., et al., 2001. Proc Natl Acad Sci U S A 98(23):13306. 6. Haynes, N.M., et al., 2007. J. Immunol. 179:5099. 7. Oliver, JA., et al. 2009. J. Am Soc Nephrol. PubMed
C57BL/6 mouse splenocytes stained with 30-F11 PE
Description:
CD45 is a 180-240 kD glycoprotein, also known as the leukocyte common antigen (LCA), T200 or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell, as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Other Names:
T200, Ly-5, LCA
Structure:
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution:
All hematopoietic cells except mature erythrocytes and platelets
Function:
Phosphatase, T and B cell activation
Ligand Receptor:
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Antigen References:
1. Barclay, A., et al., 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Trowbridge, I.S., et al., 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara, K., et al., 1993. Cell 74:143. 4. Pulido, R., et al., 1988. J. Immunol. 140:3851.
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