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PE anti-mouse CD4 Antibody
PE anti-mouse CD4 Antibody
116005 50 µg ¥17,000     
116006 200 µg ¥40,000     
Product Details
Clone: RM4-4
Isotype: Rat IgG2b, κ
Isotype Control:PE Rat IgG2b, κ Isotype Ctrl
Reactivity: Mouse
Immunogen: BALB/c mouse thymocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
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Application
Notes:

RM4-4 antibody does not block binding of the GK1.5 and RM4-5 antibodies to CD4 T cells. For immunohistochemistry applications, the RM4-5 (Cat. No. 100506) and GK1.5 (Cat. No. 100402) antibodies are recommended.
Application
References:

1. Bendelac A. 1995. Curr. Opin. Immunol. 7:367.
2. Norian LA and Allen PM. 2004. J. Immunol. 173:835.
C57BL/6 mouse splenocytes stained with
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) FITC and RM4-4 PE


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See PE spectral data



Description:

CD4 is a 55 kD protein, also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.
Other Names: L3T4, T4
Structure: Ig superfamily, 55 kD
Distribution: Majority of thymocytes, T cell subset
Function: TCR co-receptor, T cell activation
Ligand Receptor: MHC class II molecule
Antigen
References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.
GeneID: 12504
Latest Publications: View the latest CD4 articles on HighwirePress.com
UniProt: View information about CD4 on UniProt.org
Keywords: PE anti-mouse CD4, RM4-4, PE, L3T4, T4, Mouse, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • FITC anti-mouse CD4
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with RM4-4 FITC

  • Purified anti-mouse CD4
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with purified RM4-4, followed by anti-rat IgG FITC

  • PE anti-mouse CD4
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with CD3 (145-2C11) FITC and RM4-4 PE

  • Pacific Blue™ anti-mouse CD4
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD4 (clone RM4-4) Pacific Blue™ (filled histogram) or rat IgG2b, κ Pacific Blue™ isotype control (open histogram).

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