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The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for relevant formats) include: Western blotting1.
Application References:
1. Arase N, et al. 2005. Int. Immunol. 17:1227. (WB)
C57BL/6 mouse splenocytes were stimulated with immobilized TCR-β (clone H57-597) and then stained with CD8 Alexa Fluor® 647 and CD355 (clone 11-5/CRTAM) PE (top) or rat IgG2a, κ PE isotype control (bottom).
Class-I MHC-restricted T-cell associated molecule (CRTAM), designated as CD355, is an Ig domain like type I transmembrane receptor, which belongs to the nectin family. In the immune system, CRTAM is strictly expressed on activated NK cells and activated CD8-positive T cells. By binding its ligand Nectin-like molecule 2 (Necl2), CRTAM plays an important role in mediating cell adhesion and migration.
Other Names:
Class I-restricted T cell-associated molecule
Structure:
75 kD homodimer, nectin family, Ig superfamily
Distribution:
Activated NK cells and activated CD8-positive T cells
Function:
Cell adhesion, migration
Ligand Receptor:
Nectin-like molecule 2
Antigen References:
1. Takeuchi A, et al. 2009. J. Immunol. 183:4220. 2. Yeh JH, et al. 2008. Cell 132:846. 3. Arase N. 2005. Int. Immunol. 17:1227. 4. Boles KS, et al. 2005. Blood 106:779. 5. Galibert L, et al. 2005 J. Biol. Chem. 280:21955. 6. Kennedy J, et al. 2000 J. Leukoc. Biol. 67:725.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD355 (CRTAM)
C57BL/6 mouse splenocytes were stimulated with immobilized TCR-β (clone H57-597), then stained with CD8 Alexa Fluor® 647 and purified CD355 (clone 11-5/CRTAM) (top) or rat IgG2a, κ isotype control (bottom), followed by anti-rat IgG FITC.
LEAF™ Purified anti-mouse CD355 (CRTAM)
C57BL/6 mouse splenocytes were stimulated with immobilized TCR-β (clone H57-597), then stained with CD8 Alexa Fluor® 647 and purified CD355 (clone 11-5/CRTAM) (top) or rat IgG2a, κ isotype control (bottom), followed by anti-rat IgG FITC.
PE anti-mouse CD355 (CRTAM)
C57BL/6 mouse splenocytes were stimulated with immobilized TCR-β (clone H57-597) and then stained with CD8 Alexa Fluor® 647 and CD355 (clone 11-5/CRTAM) PE (top) or rat IgG2a, κ PE isotype control (bottom).
