The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC - Quality tested
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Blasius, A.L. et al. 2006. J. Immunol. 177:3260
C57BL/6 splenocytes stained with RA3-6B2 (B220) APC and 927 PE
Description:
CD317, known as BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, or PDCA-1, is type II transmembrane glycoprotein with a molecular mass of 29-33 kDa. It is predominantly expressed on Type I IFN-producing cells (IPCs) in naïve mice, but is up-regulated on most cell types following stimulation with type I IFNs and IFN-gamma. It is highly expressed on terminally differentiated normal plasmacytoid dendritic cells and some tumor cells, such as multiple myeloma, renal cell carcinoma, and melanoma cells. BST2 is a recently identified, IFN-induced cellular response factor that blocks release of HIV-1 and other retroviruses from infected cells. BST2 has been found to be the natural ligand of ILT7 in human model.
Other Names:
BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, PDCA-1
Structure:
Type II transmembrane glycoprotein with a molecular mass of 29-33 kDa.
Distribution:
Expressed on type I IFN-producing cells, plasmacytoid dendritic cells, and neoplastic B cells, such as multiple myeloma.
Function:
Recently identified antiviral protein that blocks the release of nascent retrovirus or other particles from infected cells.
Antigen References:
1. Douglas, J.L. et al. 2009. J Virol. 83(16): 7931 2. Cao, W. et al. 2009. J Exp Med. 206(7):1603 3. Neil, S.J. et al. 2008. Nature. 451:425
