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The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen or formalin embedded tissue1.
Application References:
1. McCartney S, et al. 2009. J. Exp. Med. 13:2967. (IHC)
C57BL/6 mouse splenocytes were surface stained with CD4/CD19/Ly-6G/NK1.1 PerCP, CD11c APC and CD8 FITC, followed by intracellular staining with TLR3 (clone 11F8) PE (top) or rat IgG2a, κ PE isotype control (bottom). The data was analyzed by gating on CD4/CD19/Ly-6G/NK1.1 negative and CD8 positive cells.
Toll-like receptor 3 (TLR3), also known as CD283, is type I transmembrane protein that belongs to the TLR family. It forms a large horseshoe shape that contacts with a neighboring horseshoe, forming a dimer of two horseshoes. It is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. TLR3 is expressed selectively on the cytoplasmic membrane and intracellularly in dendritic cells. It is also highly expressed in the placenta, pancreas, heart, liver, lung, and muscle. TLR3 is a pattern recognition receptor that participates in innate immune response to microbial pathogens by recognizing polyinosine-polycytidylic acid (Poly(I:C)) and dsRNA. Ligand binding by TLR3 induces receptor dimerization which results in inducing NF-κB activation (via TRIF-linked RIP1/TLR3 interactions) and cytokine production. TLR3 has been shown to interact with a number of proteins including MYD88, TRAF6, TRIAD3, MAP3K7, and TAB2.
Other Names:
Toll like receptor 3
Structure:
Toll-like receptor family member; type I membrane protein containing multiple leucine repeats and a interleukin-1 receptor like region in the extracellular domain. Predicted molecular weight approximately 116 kD.
Distribution:
Selective expression in dendritic cells. Highly expressed in placenta and the pancreas; also expressed in heart, liver, lung and muscle.
Function:
Pattern recognition receptor that participates in innate immune response to microbial pathogens. TLR3 ligands induce NF-κB (via TRIF-linked RIP1/TLR3 interactions) and cytokine production. TLR3 stimulation results in the production of cytokines.
Ligand Receptor:
Polyinosine-polycytidylic acid (Poly(I:C)), dsRNA
Interaction:
MYD88, TRAF6, TRIAD3, MAP3K7, and TAB2
Antigen References:
1. Alexopoulou L, et al. 2001. Nature 413:732. 2. Doyle SE, et al. 2002. Immunity 17:251. 3. Meylan E, et al. 2004. Nat Immunol. 5:503. 4. Muzio M, et al. 2000. J. Immunol. 164:5998. 5. Rock FL, et al. 1998. P. Natl. Acad. Sci. USA 95:588.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD283 (TLR3)
C57BL/6 mouse splenocytes were surface stained with CD4/CD19/Ly-6G/NK1.1 PerCP, CD11c APC and CD8 FITC, followed by intracellular staining with TLR3 (clone 11F8) PE (top) or rat IgG2a, κ PE isotype control (bottom). The data was analyzed by gating on CD4/CD19/Ly-6G/NK1.1 negative and CD8 positive cells.
PE anti-mouse CD283 (TLR3)
C57BL/6 mouse splenocytes were surface stained with CD4/CD19/Ly-6G/NK1.1 PerCP, CD11c APC and CD8 FITC, followed by intracellular staining with TLR3 (clone 11F8) PE (top) or rat IgG2a, κ PE isotype control (bottom). The data was analyzed by gating on CD4/CD19/Ly-6G/NK1.1 negative and CD8 positive cells.
