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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: blocking of ligand binding. The LEAF™ format is suggested for blocking studies.
Application References:
1. Akbari O, et al. 2002. Nat. Med. 8:1024. 2. Harada H, et al. 2003. J. Clin. Invest. 112:234. 3. McAdam AJ, et al. 2000. J. Immunol. 165:5035. (FC Block) 4. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
Con A-stimulated (3 days) C57BL/6 splenocytes stained with 7E.17G9 PE.
The 7E.17G9 antibody reacts with the 47-57 kD ICOS protein, also known as inducible costimulatory molecule, and H4. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes and can costimulate T cells and induce proliferation. In addition ICOS has been shown to be involved in humoral immune responses (B cell germinal center formation). The ICOS ligand, B7h/B7RP-1 and B7-H2 is constitutively expressed in B cell areas of secondary lymphoid organs and can be induced in other tissues by LPS. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development. ICOS stimulation has been shown to be involved in airway tolerance and the downregulation of pulmonary inflammation.
Other Names:
Inducible COStimulatory molecule, H4
Structure:
CD28/CTLA-4, 47-57kD
Distribution:
Activated T cells, subset of thymocytes
Function:
Costimulates T cell activation, proliferation, humoral immune response
Ligand Receptor:
B7h/B7RP-1/GL-50
Antigen References:
1. Rudd CE, et al. 2003. Nat. Rev. Immunol. 3:544. 2. McAdam AJ, et al. 2000. J. Immunol. 165:5035. 3. Mak TW, et al. 2003. Nat. Immunol. 4:765
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD278 (ICOS)
Con A-stimulated (3 days) Balb/c splenocytes stained with biotinylated 7E.17G9, followed by Sav-PE
LEAF™ Purified anti-mouse CD278 (ICOS)
Con A-stimulated (3 days) C57BL/6 splenocytes stained with 7E.17G9 PE
PE anti-mouse CD278 (ICOS)
Con A-stimulated (3 days) C57BL/6 splenocytes stained with 7E.17G9 PE.
