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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: in vitro and in vivo induction of cytotoxicity2,3. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 119908).
Application References:
1. Hayakawa Y, et al. 2004. J. Immunol. 172:123. (FC) 2. Takeda K, et al. 2004. J. Exp. Med. 199:437. (Cyt) 3. Sato K, et al. 2005. J. Immunol. 174:4025. (Cyt) 4. Iannello A, et al. 2009. J. Virol. 83:5999. PubMed 5. Grisendi G, et al. 2010. Cancer Res. 70:3718. PubMed
CD262 is a 55 kD protein also known as DR5, TRAIL-R2, Apo-2, TRICK2, and KILLER. DR5 is member 10B of the TNF receptor superfamily (TNFRSF10B), widely expressed in normal tissues and some tumor cell lines. The DR5 receptor binds to TRAIL and interacts with the cytoplasmic proteins TRADD and RIP. TRAIL binding to DR5 activates NF- κB and induces apoptosis. DR5 is induced by wild-type p53.
Other Names:
DR5, TRAIL-R2, TNFRS10B, TRICK2, KILLER
Structure:
Type I transmembrane protein, TNF receptor superfamily member, homotrimer, predicted molecular weight 55 kD
Distribution:
Widely expressed in normal tissues and some tumor cell lines. Expression regulated by the tumor suppressor p53
Function:
Binds to TRAIL, activates NF- ?B, induces apoptosis. May be involved in the regulation of tumor metastases.
Ligand Receptor:
TRAIL (CD253); cytoplasmic domain interacts with TRADD and RIP
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
LEAF™ Purified anti-mouse CD262 (DR5, TRAIL-R2)
Mouse DR5 transfected cells stained with LEAF™ purified MD5-1, followd by biotinylated anti-Armenian hamster IgG and Sav-PE
