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PE anti-mouse CD244.2 (2B4 B6 Alloantigen) Antibody
PE anti-mouse CD244.2 (2B4 B6 Alloantigen) Antibody
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Lee KM, et al. 2006. Blood Apr 15:107:3181. 2. Schatzle JD, et al. 1999. P. Natl Acad Sci USA 96:3870. 3. Stepp SE, et al. 1999. Eur. J. Immunol. 29:2392. 4. de la Luz Sierra M, et al. 2010. Blood 115:3970. PubMed
C57BL/6 splenocytes stained with m2B4(B6)458.1 PE (upper panel) or mouse IgG1 isotype control PE (lower panel) and anti-mouse CD49b (DX5) APC
Mouse CD244, also known as 2B4, is a receptor belonging to the CD2 family of proteins involved in non- MHC-restricted cytotoxicity. It is expressed on all natural killer (NK) cells, IL-2 activated NK (LAK) cells, NKT cells, and a subset of T lymphocytes, including dendritic epidermal T cells. At least there are two isoforms of CD244.2 proteins that differ in the length of the cytoplasmic domain. The long form functions in an inhibitory manner, while the short form functions in an activating manner. The ligand of CD244 is CD48, which expressed on all hematopoietic cells. It was reported that CD244 interacts with CD48 is essential for IL-2-driven expansion and activation of murine NK cells. The 2B4 antibody reacts with CD244.2, the 2B4 allloantigen which is expressed on C57BL/6 mice, but not on Balb/c mice.
Other Names:
2B4 B6 Alloantigen
Structure:
A member of the CD2 subset of the immunoglobulin superfamily (CD2 IgSF).
Distribution:
All natural killer (NK) cells, IL-2 activated NK (LAK) cells, NKT cells, and a subset of T lymphocytes
Function:
Mediate non-MHC-restricted cytotoxicity.
Ligand Receptor:
CD48
Antigen References:
1. Brown MH, et al. 1998. J. Exp. Med. 188:2083. 2. Davis SJ, et al. 1996. Immunol Today. 17:177. 3. Kumaresan PR, et al. 2000. Immunogenetics. 51:306. 4. Latchman Y, et al. 1998. J. Immunol. 161:5809.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD244.2 (2B4 B6 Alloantigen)
C57BL/6 splenocytes stained with m2B4(B6)458.1 FITC and NK-1.1 APC
FITC anti-mouse CD244.2 (2B4 B6 Alloantigen)
C57BL/6 splenocytes stained with m2B4(B6)458.1 FITC and NK-1.1 APC
Biotin anti-mouse CD244.2 (2B4 B6 Alloantigen)
C57BL/6 splenocytes stained with m2B4 (B6) 458.1 biotin (upper panel) or mouse IgG1 isotype control biotin (lower panel) followed by Sav-PE and anti-mouse CD49b DX5 APC
PE anti-mouse CD244.2 (2B4 B6 Alloantigen)
C57BL/6 splenocytes stained with m2B4(B6)458.1 PE (upper panel) or mouse IgG1 isotype control PE (lower panel) and anti-mouse CD49b (DX5) APC
C57BL/6 splenocytes stained with m2B4(B6)458.1 Alexa Fluor® 647 (upper panel) or mouse IgG1 isotype control Alexa Fluor® 647 (lower panel) and anti-mouse CD49b (DX5) PE
