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PE anti-mouse CD2 Antibody
PE anti-mouse CD2 Antibody
100107 50 µg $90.00 Qty:
    
100108 200 µg $250.00 Qty:
    

Product Details

 
Clone: RM2-5 (See other available formats)
Isotype: Rat IgG2b, λ
Reactivity: Mouse
Immunogen: BALB/c mouse thymocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation
Laser:
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
COA:
Enter Lot#:   
Application
Notes:

Additional reported applications (for the relevant formats) include: in vitro blocking of CD2-mediated cell-cell adhesion and inhibition of T cell activation1, blocking of T cell A.I.C.D.2, immunoprecipitation3, and co-induction of thymocyte maturation4. The RM2-5 antibody can block CD2-mediated cell-cell adhesion. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 100110).

Application
References:

  Publication Library
PE anti-mouse CD2 Antibody RM2-5 Image 1
C57BL/6 mouse splenocytes stained with RM2-5 PE


Compare all formats

See PE spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

CD2 is a 45-58 kD type I transmembrane glycoprotein, also known as LFA-2, T11 or Ly-37. It is a member of the Ig superfamily. Mouse CD2 is primarily expressed on T cells, B cells, thymocytes and NK cells. It is a ligand for CD48 and is involved in T cell activation and differentiation.

Other Names: LFA-2, T11, Ly-37, SRBC-R
Structure: Ig superfamily, 45-58 kD
Distribution: T cells, thymocytes, NK cells, B cells
Function: T cell activation, adhesion
Ligand Receptor: CD48, lck
Antigen
References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis SJ, et al. 1996. Immunol. Today 17:177.
3. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.

GeneID: 12481
Latest Publications: View the latest CD2 articles on HighwirePress.com
UniProt: View information about CD2 on UniProt.org
Keywords: PE anti-mouse CD2, RM2-5, PE, LFA-2, T11, Ly-37, SRBC-R, Mouse, Flow Cytometry, Immunology, Antibodies


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Other Formats

DescriptionCloneApplications
APC anti-mouse CD2RM2-5FC
Biotin anti-mouse CD2RM2-5FC
FITC anti-mouse CD2RM2-5FC
LEAF™ Purified anti-mouse CD2RM2-5Block, Costim, FC, IP
PE/Cy7 anti-mouse CD2RM2-5FC
PerCP/Cy5.5 anti-mouse CD2RM2-5FC
Purified anti-mouse CD2RM2-5FC, IP


Related Pathways

Pathway
ATM Pathway
TH1 Pathway (Cellular Immune Response)
TH2 Pathway (Humoral Immune Response)


Related Categories

Category
Immunology > Mouse Immunology > Stem Cells > CD2
Immunology > Mouse Immunology > B Cells > CD2
Immunology > Mouse Immunology > NK/NKT Cells > CD2
Immunology > Mouse Immunology > CD and Related Molecules > CD2 > RM2-5
Immunology > Mouse Immunology > Costimulatory Molecules > CD2 > RM2-5

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-mouse CD2
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with biotinylated RM2-5, followed by Sav-PE

  • FITC anti-mouse CD2
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with RM2-5 FITC

  • PE anti-mouse CD2
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with RM2-5 PE

  • LEAF™ Purified anti-mouse CD2
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with LEAF™ purified RM2-5, followed by anti-rat IgG FITC

  • Purified anti-mouse CD2
     C57BL/6 mouse splenocytes stained

    C57BL/6 mouse splenocytes stained with purified RM2-5

  • APC anti-mouse CD2
    C57BL/B6 mouse splenocytes were stained

    C57BL/B6 mouse splenocytes were stained with CD2 (clone RM2-5) APC (filled histogram) or rat IgG2b, λ APC isotype control (open histogram).

  • PE/Cy7 anti-mouse CD2
    C57BL/B6 mouse splenocytes were stained

    C57BL/B6 mouse splenocytes were stained with CD2 (clone RM2-5) PE/Cy7 (filled histogram) or rat IgG2b, κ PE/Cy7 isotype control (open histogram).

  • PerCP/Cy5.5 anti-mouse CD2
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD2 (clone RM2-5) PerCP/Cy5.5 (filled histogram) or rat IgG2b, κ PerCP/Cy5.5 isotype control (open histogram).

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