Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional reported applications (for the relevant formats) include: in vitro blocking of CD2-mediated cell-cell adhesion and inhibition of T cell activation1, blocking of T cell A.I.C.D.2, immunoprecipitation3, and co-induction of thymocyte maturation4. The RM2-5 antibody can block CD2-mediated cell-cell adhesion. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 100110).
CD2 is a 45-58 kD type I transmembrane glycoprotein, also known as LFA-2, T11 or Ly-37. It is a member of the Ig superfamily. Mouse CD2 is primarily expressed on T cells, B cells, thymocytes and NK cells. It is a ligand for CD48 and is involved in T cell activation and differentiation.
LFA-2, T11, Ly-37, SRBC-R
Ig superfamily, 45-58 kD
T cells, thymocytes, NK cells, B cells
T cell activation, adhesion
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis SJ, et al. 1996. Immunol. Today 17:177. 3. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD2
C57BL/6 mouse splenocytes stained with biotinylated RM2-5, followed by Sav-PE
FITC anti-mouse CD2
C57BL/6 mouse splenocytes stained with RM2-5 FITC
PE anti-mouse CD2
C57BL/6 mouse splenocytes stained with RM2-5 PE
LEAF™ Purified anti-mouse CD2
C57BL/6 mouse splenocytes stained with LEAF™ purified RM2-5, followed by anti-rat IgG FITC
Purified anti-mouse CD2
C57BL/6 mouse splenocytes stained with purified RM2-5
APC anti-mouse CD2
C57BL/B6 mouse splenocytes were stained with CD2 (clone RM2-5) APC (filled histogram) or rat IgG2b, λ APC isotype control (open histogram).
PE/Cy7 anti-mouse CD2
C57BL/B6 mouse splenocytes were stained with CD2 (clone RM2-5) PE/Cy7 (filled histogram) or rat IgG2b, κ PE/Cy7 isotype control (open histogram).
PerCP/Cy5.5 anti-mouse CD2
C57BL/6 mouse splenocytes were stained with CD2 (clone RM2-5) PerCP/Cy5.5 (filled histogram) or rat IgG2b, κ PerCP/Cy5.5 isotype control (open histogram).