The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Krug, A., et al., 2002. J. Immunol. 169:6079. 2. McAleer, JP., et al. 2009. J. Immunol. 182:5322. PubMed 3. Sharma, R., 2009 J. Immunol. 183:3212 (FC) PubMed 4. Barbi, J., et al., 2009. FASEB J. PubMed 5. Kimpfler, S., et al., 2009. J. Immunol. 183:6330. PubMed
C57BL/6 splenocytes stained with CXCR3-173 PE and B220 FITC
Description:
CD183, also known as CXCR3, is a member of C-X-C chemokine family characterized by a pair of cysteine residues separated by a single amino acid. CXCR3 is a 38 kD seven pass transmembrane receptor coupled to G-protein. It mediates Ca2+ mobilibization and chemotaxis in response to C-X-C chemokines, such as IP10 (CXCL10), MIG (CXCL9), I-TAC (CXCL11) and PF4 (CXCL4). CXCR3 is expressed primarily on activiated T lymphocytes, NK cells, and some epithelial cells and endothelial cells. It is not expressed on B cells, monocytes or granulocytes.
Essential in T cell recruitment to sites of inflammation
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
