The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1.Krug, A., et al., 2002.J. Immunol. 169:6079. 2. Barbi, J. et al. 2009. FASEB J. PubMed 3. Kimpfler, S., et al. 2009. J. Immunol. 183:6330. PubMed.
C57BL/6 splenocytes stained with CXCR3-173 PE and B220 FITC
Description:
CD183, also known as CXCR3, is a member of C-X-C chemokine family characterized by a pair of cysteine residues separated by a single amino acid. CXCR3 is a 38 Kd seven pass transmembrane receptor coupled to G-protein . It mediates Ca2+ mobilibization and chemotaxis in response to C-X-C chemokines, such as IP10(CXCL10), MIG (CXCL9), I-TAC (CXCL11) and PF4 (CXCL4). CXCR3 is expressed primarily on activiated T lymphocytes, NK cells, and some epithelial cells and endothelial cells. It is not expressed on B cells, monocytes or granulocytes.
