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The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining in frozen tissue sections1 and immunofluorescence microscopy1,2.
Application References:
1. Barral P, et al. 2010. Nat. Immunol. 11:303. (IHC, IF) 2. Chtanova T, et al. 2008. Immunity 29:487. (IF)
C57BL/6 splenocytes were stained with F4/80 APC, Ly-6G PerCP, and CD169 (clone 3D6.112) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data was analyzed by gating on Ly-6G-negative cell population.
CD169, also known as Siglec-1 and Sialoadhesin (Sn), is a type I lectin containing 17 immunoglobulin (Ig) domains (one variable domain and 16 constant domains). CD169 binds to sialic acids, which can be found on PSGL-1, CD43, CD206, and CD227. By its affinity to α2, 3-linked sialic acid, it is involved in macrophage binding to different cell types such as granulocytes, monocytes, NK, B, and T cells. CD169 was initially identified as a sialic acid-dependent sheep erythrocyte receptor (SER) on resident bone marrow cells of mice. Recently, it has been identified as highly expressed on resident bone marrow macrophages which plays an important role in retention of stem cells in mesenchymal stem cell niche. It is also found on some specific subsets of tissue macrophages in spleen, lymph nodes, bone marrow, liver, colon, lungs, and cancer cells. Recent evidences suggest that CD169-positive macrophages serve as lymph node-resident APCs to dominate early activation of tumor antigen-specific CD8-positive cells and invariant NK cell.
Type I single membrane-spanning lectin containing 17 immunoglobulin (Ig) domains, belongs to the immunoglobulin superfamily.
Distribution:
Macrophages in spleen, lymph nodes, bone marrow, liver, colon and lungs.
Function:
Adhesion.
Ligand Receptor:
PSGL-1, CD43, CD206 and CD227.
Antigen References:
1. Chow A, et al. 2011. J. Exp. Med. 208:261. 2. Asano K, et al. 2011. Immunity 34:85. 3. Xiong YS, et al. 2009. Clin. Biochem. 42:1057. 4. Varki A, et al. 2009. Glycoconj. J. 26:231. 5. Rempel H, et al. 2008. PLoS One 3:e1967. 6. Crocker PR, et al. 2001. Trends Immunol. 22:337. 7. Hartnell A, et al. 2001. Blood 97:288. 8. Crocker PR, et al. 1985. J. Exp. Med. 162:993.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD169 (Siglec-1)
C57BL/6 splenocytes were stained with F4/80 APC, Ly-6G PerCP, and CD169 (clone 3D6.112) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data was analyzed by gating on Ly-6G-negative cell population.
PE anti-mouse CD169 (Siglec-1)
C57BL/6 splenocytes were stained with F4/80 APC, Ly-6G PerCP, and CD169 (clone 3D6.112) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data was analyzed by gating on Ly-6G-negative cell population.
