Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: in vitro blocking2 of cell-cell adhesion and immunohistochemical staining1 of acetone-fixed frozen sections. The 2D7 antibody does not block the binding of M17/4 antibody (Cat. No. 101102) to CD11a.
Application References:
1. Kootstra CJ, et al. 1997. Clin. Exp. Immunol. 108:324. (IHC) 2. Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block) 3. Masten BJ, et al. 1999. J. Immunol. 162:1310. (FC) 4. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed 5. Cullere X, et al. 2008. Blood 111:3591. PubMed
CD11a is a 180 kD glycoprotein, also known as αL integrin, LFA-1 α, Ly-15, or Ly-21. It is a member of the integrin family, primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes. In association with CD18, the CD11a/CD18 complex forms LFA-1. CD11a plays an important role in intercellular adhesion and costimulation by binding its ligands, ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50). The 2D7 antibody can block CD11a-mediated cellular adhesion.
Other Names:
αL integrin, LFA-1 α subunit, Ly-15, Ly-21
Structure:
Integrin family, associates with integrin ß2 (CD18), 180 kD
Distribution:
Lymphocytes, granulocytes, monocytes, macrophages
Function:
Cellular adhesion
Ligand Receptor:
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50)
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Springer TA. 1994. Cell 76:301. 3. Lub M, et al. 1995. Immunol. Today 16:479.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-mouse CD11a
CD57BL/6 mouse splenocytes stained with 2D7 FITC
PE anti-mouse CD11a
CD57BL/6 mouse splenocytes stained with 2D7 PE
Purified anti-mouse CD11a
C57BL/6 mouse splenocytes stained with purified 2D7, followed by anti-rat IgGs-FITC
