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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining1,2 of acetone-fixed frozen sections, and immunofluorescence microscopy3.
Application References:
1. Chen JW, et al. 1985. Arch. Biochem. Biophys. 239:574. 2. Chen JW, et al. 1986. Biochem. Soc. Symp. 51:97. 3. Rohrer J, et al. 1996. J. Cell Biol. 132:565. 4. Steptoe RJ, et al. 2007. J. Immunol. 178:2094.
Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with 1D4B PE
CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. The mature CD107a is heavily glycosylated from a 40 kD core protein. It is expressed by macrophages, epithelial cells, endothelial cells, and some tumor cells. This molecule is located on the luminal side of lysosomes and has been suggested to play a role in the protection of lysosome membrane from lysosomal hydrolases. CD107a is a ligand for galaptin. Upon activation, CD107a is transferred to the surface of cell membrane. It plays a role in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression.
Other Names:
Lysosome-Associated Membrane Protein 1, LAMP-1, LGP-120
Structure:
Type I transmembrane glycoprotein, 40 kD core protein, mature protein is heavily glycosylated with 110-140 kD
Distribution:
Macrophages, epithelial cells, endothelial cells, some tumor cells, located on the luminal side of lysosomes or on the surface of cell membrane
Function:
Protect lysosomal membrane from lysosomal hydrolases, adhesion
Ligand Receptor:
Galaptin
Antigen References:
1. Barclay AN, et al. 1997. The Leucocyte Antigen Facts Book. 2nd edition. Academic Press. 2. Heffernan M, et al. 1989. Cancer Res. 49:6077. 3. Granger BL, et al. 1990. J. Bio. Chem. 265:12036. 4. De Carvalho Bittencourt M, et al. 2005. Eur. J. Immunol. 35:1501.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-mouse CD107a (LAMP-1)
Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with 1D4B PE
Purified anti-mouse CD107a (LAMP-1)
Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with purified 1D4B, followed by anti-rat IgG FITC
Biotin anti-mouse CD107a (LAMP-1)
Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with biotinylated 1D4B, followed by Sav-PE
FITC anti-mouse CD107a (LAMP-1)
Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with 1D4B FITC
