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The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser:
Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm)
B7-H4 is a newly discovered B7 family member that negatively regulates T cell immunity. In vitro, B7-H4 inhibits CD4+ and CD8+ T cell proliferation, cytokine production, and generation of alloreactive cytotoxic T-cells (CTLs). In vivo, blockage of endogenous B7-H4 by specific monoclonal antibodies promotes T cell responses. A new reported function of B7-H4 is as an important negative regulator of innate immunity through growth inhibition of neutrophils. It has been reported that B7-H4 is not constitutively expressed on peripheral tissues but can be induced to express on T cells, B cells, macrophages, and dendritic cells. B7-H4 is expressed on some tumor cancer cells. The role of B7-H4 in tumor progression may be to transform precancerous cells and then protect them from immunosurveillance. Although B- and T-lymphocyte attenuator (BTLA) was proposed to be the receptor for B7-H4, further studies are needed to identify the inhibitory receptor of B7-H4.
Other Names:
B7x
Structure:
Belong to the B7 family, contain two extracellular Ig-like domains.
Distribution:
Induced to express on T cells, B cells, macrophages, and dendritic cells. It is also expressed on some tumor cancer cells.
Function:
Inhibit T cell activation, cell cycle progression, and cytokine production.
Antigen References:
1. Choi IH, et al. 2003. J. Immunol. 171:4650. 2. Prasad DV, et al. 2003. Immunity 18:863. 3. Kryczek I, et al. 2006. J. Exp. Med. 203:871.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse B7-H4 (B7S1, B7X)
Mouse B7-H4 transfected 293 cells stained with purified HMH4-5G1 conjugated with PE
LEAF™ Purified anti-mouse B7-H4 (B7S1, B7X)
Mouse B7-H4 transfected 293 cells stained with purified HMH4-5G1 conjugated with PE
PE anti-mouse B7-H4 (B7S1, B7X)
Mouse B7-H4 transfected 293 cells stained with HMH4-5G1 PE
