The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC - Quality tested
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Mouse B7-H4 transfected 293E cells stained with clone 9 PE
Description:
B7-H4 is a newly discovered B7 family member that negatively regulates T cell immunity by inhibiting of T cell proliferation, cytokine production, and cell cycle progression. In vitro, B7-H4 inhibits CD4+ and CD8+ T cell proliferation, cytokine production, and generation of alloreactive cytotoxic T-cells (CTLs). In vivo, blockade of endogenous B7-H4 by specific monoclonal antibody promotes T cell responses. A new function of B7-H4 was reported as an important negative regulator of innate immunity through growth inhibition of neutrophils. It was reported that B7-H4 does not constitutively expressed on peripheral tissues but could be induced to express on T cells, B cells, macrophages, and dendritic cells. B7-H4 is expressed on some tumor cancer cells. The role of B7-H4 in tumor progression may be to transform precancerous cells and then protect them from immunosurveillance. Although B- and T- lymphocyte attenuator (BTLA) was proposed to be the receptor for B7-H4, further studies are needed to identify the inhibitory receptor of B7-H4.
Other Names:
B7H4, B7S1, B7-S1, B7x
Structure:
Belong to the B7 family of costimulatory proteins and contain two extracellular Ig-like domains.
Distribution:
Induced to express on T cells, B cells, macrophages, and dendritic cells. It is also expressed on some tumor cancer cells.
Function:
Inhibit T cell activation, cell cycle progression, and cytokine production.
