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PE anti-human Tim-4 Antibody
PE anti-human Tim-4 Antibody
354003 25 tests $125.00 Qty:
    
354004 100 tests $295.00 Qty:
    

Product Details

 
Clone: 9F4 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:PE Mouse IgG1, κ Isotype Ctrl (FC)
Reactivity: Human
Immunogen: Human TIM-4- IgV Fc, fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation
Laser:
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
COA:
Enter Lot#:   
Application
Notes:

Clone 9F4 recognizes the FG loop of human TIM-4.1

Application
References:

  Publication Library
PE anti-human Tim-4 Antibody 9F4 Image 1
Human Tim-4 transfected CHO cells were stained with TIM-4 (clone 9F4) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).


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See PE spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th-1 and Th-2) cell-mediated immunity. Tim-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of Tim-1. This binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, TIM-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The TIM-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and is connected to TIM-1-triggered Th2 responsiveness.

Other Names: SMUCKLER (Spleen, mucin-containg, knock-out of lymphotoxin (LT)), TIMD4 (T-cell immunoglobulin and mucin domain containing 4)
Structure: Type 1 transmembrane glycoprotein and mucin domain containing protein 4.
Distribution: Splenic CD14+, macrophages, immature DC.
Function: TIM-1 ligation to TIM-4 induces T cell proliferation, promotes cytokine production, and aids in the regulation of Th2 immunity.
Ligand Receptor: Phosphatiylserine is the receptor of TIM4, and TIM4 is a ligand of TIM1.
Antigen
References:

1. Kobayashi N, et al. 2007. Immunity 27:927.
2. Meyers JH, et al. 2005. Nat. Immunol. 6:455.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Shakhov AN, et al. 2004. Eur. J. Immunol. 34:494.

GeneID: 91937
Latest Publications: View the latest Tim-4 articles on HighwirePress.com
UniProt: View information about Tim-4 on UniProt.org
Keywords: PE anti-human Tim-4, 9F4, PE, SMUCKLER (Spleen, mucin-containg, knock-out of lymphotoxin (LT)), TIMD4 (T-cell immunoglobulin and mucin domain containing 4), Human, Flow Cytometry, Immunology, Antibodies


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Other Formats

DescriptionCloneApplications
Purified anti-human Tim-49F4FC


Related Pathways

Pathway


Related Categories

Category
Immunology > Human Immunology > Costimulatory Molecules > Tim-4
Immunology > Human Immunology > Th17 > Tim-4
Immunology > Human Immunology > Th1/Th2 > Tim-4
Immunology > Human Immunology > Monocyte/Macrophage > Tim-4
Immunology > Human Immunology > Dendritic cells > Tim-4
Immunology > Human Immunology > CD and Related Molecules > Tim-4

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 2 Revision Date: 2013-05-15
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human Tim-4
    Human Tim-4 transfected CHO cells

    Human Tim-4 transfected CHO cells were stained with purified Tim-4 (clone 9F4) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.

  • PE anti-human Tim-4
    Human Tim-4 transfected CHO cells

    Human Tim-4 transfected CHO cells were stained with TIM-4 (clone 9F4) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).

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