Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm)
The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th-1 and Th-2) cell-mediated immunity. Tim-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of Tim-1. This binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, Tim-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The Tim-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and is connected to Tim-1-triggered Th2 responsiveness.
SMUCKLER (Spleen, mucin-containg, knock-out of lymphotoxin (LT)), TIMD4 (T-cell immunoglobulin and mucin domain containing 4)
Type 1 transmembrane glycoprotein and mucin domain containing protein 4.
Splenic CD14+, macrophages, immature DC.
Tim-1 ligation to Tim-4 induces T cell proliferation, promotes cytokine production, and aids in the regulation of Th2 immunity.
Phosphatiylserine is the receptor of Tim-4, and Tim-4 is a ligand of Tim-1.
1. Kobayashi N, et al. 2007. Immunity 27:927. 2. Meyers JH, et al. 2005. Nat. Immunol. 6:455. 3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454. 4. Shakhov AN, et al. 2004. Eur. J. Immunol. 34:494.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human Tim-4
Human Tim-4 transfected CHO cells were stained with purified Tim-4 (clone 9F4) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
PE anti-human Tim-4
Human Tim-4 transfected CHO cells were stained with TIM-4 (clone 9F4) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
APC anti-human Tim-4
Human Tim-4 transfected CHO cells were stained with TIM-4 (clone 9F4) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
PE/Cy7 anti-human Tim-4
Human Tim-4 transfected CHO cells were stained with TIM-4 (clone 9F4) PE/Cy7 (filled histogram) or mouse IgG1, κ PE/Cy7 isotype control (open histogram).