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The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunofluorescence staining2, Western blotting2, immunoprecipitation2, and ELISA3.
Application References:
1. Zhang J, et al. 2000. J. Biol. Chem. 275:22121. 2. Avril T, et al. 2004. J. Immunol. 173:6841. (IF, IP, WB) 3. Biedermann B, et al. 2007. Leukemia Res. 31:221. (ELISA)
Human peripheral blood granulocytes were stained with Siglec-9 (clone K8) PE (filled histogram) or mouse IgG1 PE isotype control (open histogram).
Siglecs are cell surface receptors belonging to the immunoglobulin superfamily that recognize sugar antigens. The extracellular domain of siglec-9 contains an IgV region, which binds sialic acid, followed by two IgC regions. Siglec 9 and siglec 6-8,10-12 are CD33 (siglec 3) like siglecs, which have two ITIMs in the cytoplasmic tails, suggesting their functional involvement in signal transduction. It is highly expressed on neutrophils and monocytes, and at lower levels on the subpopulations of T and B lymphocytes and NK cells. Siglec-9 plays a role in negative regulation of T cell activation, and it also affects neutrophil apoptosis.
Other Names:
Sialic acid-binding Ig-like lectin-9
Structure:
Immunoglobulin superfamily
Distribution:
Highly expressed on neutrophils and monocytes, and at lower levels on the subpopulations of T and B lymphocytes and NK cells
Ligand Receptor:
Sialic acid
Antigen References:
1. Ikehara Y, et. al. 2004. J. Biol. Chem. 279:43117. 2. von Gunten S, et al. 2005. Blood 106:1423.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human Siglec-9
Human peripheral blood granulocytes were stained with purified Siglec-9 (clone K8) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
PE anti-human Siglec-9
Human peripheral blood granulocytes were stained with Siglec-9 (clone K8) PE (filled histogram) or mouse IgG1 PE isotype control (open histogram).
