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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Human cervical cancer cell line, HeLa, stained with W7C6 PE
Human bone marrow-derived mesenchymal stem cells stained with W7C6 PE
The W7C6 antibody was generated by immunization with Weri-RB-1 cells. It reacts with mesenchymal stem cells and some tested neoplastic cell lines (HT29, Hela, MCF-7, REH). Further studies have shown that W7C6 antigen is identical to protein tyrosine phosphatase LAR. Mesenchymal stem cells or MSCs are originally name as bone marrow stromal cells. They are a population of adult stem cells with a large capacity for self-renewal and multipotency for differentiation into a variety of cell types including osteoblasts, chondrocytes, myocytes, adipocytes, β-pancreatic islets cells and certain neuronal cells. MSCs reside in many tissues, such as bone marrow, placenta, adipose tissue, adult peripheral blood, fetal blood, skin, as well as liver and lung. Besides their plasticity for tissue repair, MSCs also exhibit a powerful immunosuppressive activity and play important role in supporting hematopoiesis.
1. Buhring HJ, et al. 2007. Ann. N. Y. Acad. Sci. 1106:262. 2. Jackson L, et al. 2007 J. Postgrad. Med. 53:121. 3. Krampera M, et al. 2003 Blood 101:3722. 4. Ball LM, et al. 2007 Blood 110:2764
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human MSC (W7C6)
Human pre-B leukemia cell line REH stained with purified anti-mouse FITC
Biotin anti-human MSC (W7C6)
Human bone marrow-derived mesenchymal stem cells stained with biotinylated W7C6, followed by Sav-PE
PE anti-human MSC (W7C6)
Human cervical cancer cell line, HeLa, stained with W7C6 PE
Human bone marrow-derived mesenchymal stem cells stained with W7C6 PE
