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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation2, Western blotting2.
This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.
Application References:
1. Qian D, et al. 1997. J. Exp. Med. 185:1253. (ICFC) 2. Crespo M, et al. 2003. New Engl. J. Med. 348:1764. (WB IP) 3. Jamroziak K, et al. 2009. Cancer Epidemiol Biomarkers Prev. PubMed
Human peripheral blood lymphocytes intracellular stained with CD4 FITC and 1E7.2 PE
ZAP-70 is a 70 kD protein tyrosine kinase associated with the zeta chain of the T cell receptor. It is expressed in T cells and NK cells and has been shown to be involved in T cell signaling. Defects in ZAP-70 have been linked to selective T cell defects. The ZAP-70 kinase undergoes multiple phosphorylation events after T cell receptor engagement and interacts with a number of proteins involved in signal transduction. Recently, ZAP-70 has been identified as an important prognostic marker in B-cell chronic lymphocytic leukemia (B-CLL). The 1E7.2 monoclonal antibody recognizes human and mouse ZAP-70 and has been shown to be useful for flow cytometry, Western blotting, and immunoprecipitation.
Other Names:
Zeta chain associated protein kinase, SYK related tyrosine kinase
Structure:
Protein tyrosine kinase, contains two SH2 domains, 70 kD
Distribution:
T cells, NK cells, B-cell chronic lymphocytic leukemia (B-CLL)
Function:
Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated. Defects in ZAP-70 causes selective T cell defect
Ligand Receptor:
Binds to CD3 zeta chain, also associates with a variety of proteins including SLA, Fyn, RasGap, Lck, Vav1, and Shc
Modification:
Multiple phosphorylation sites (Tyr319 phosphorylation essential for full activity)
Antigen References:
1. Chan AC, et al. 1992. Cell 71:649. 2. Chan AC, et al. 1994. Science 264:1559. 3. Arpaia E, et al. 1994. Cell 76:947.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human/mouse ZAP-70
Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-ZAP-70 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
PE anti-human/mouse ZAP-70
Human peripheral blood lymphocytes intracellular stained with CD4 FITC and 1E7.2 PE
FITC anti-human/mouse ZAP-70
Human peripheral blood lymphocytes intracellularly stained with 1E7.2 FITC and CD3 APC
