The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC - Quality tested
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Human lymphoma cell line SR stained with MHJ2-523 PE
Description:
The binding of Notch ligands with their receptors results in the proteolysis of Notch and movement of intracellular portion of Notch into the nucleus. This translocation triggers a series of signaling process. The Notch receptors and their ligands are highly conserved signaling molecules throughout development. They regulate cell fate decision for various cell developments, including myogenesis, neurogenesis, and lymphocyte development. There is growing evidence that signals transmitted through different Notch ligands can differentially affect target cells. The Jagged-2 is one of the several Notch ligands identified. It is a single pass transmembrane protein consisting of a large extracellular N-terminal which includes the DSL (Delta-Serrate-Lag-2) domain, followed by a tandem array of EGF-like repeats (ELRs) and cysteine-rich domain (CRD). Jagged 2 is expressed on lymphoma cells and some other tumor cells. Jagged-2 is reported to be essential for the regulation of spermatogenesis. Notch1/Jagged-2 signaling system plays an important role for male germ cells to differentiate, T cells and B cells to differentiate.
Other Names:
Jag2, Jagged-2
Structure:
A single pass transmembrane protein consisting of a large extracellular N-terminal which includes the DSL domain, followed by a tandem array of EGF-like repeats (ELRs) and cysteine-rich domain (CRD).
Distribution:
Expressed on lymphoma cells and some other tumor cells.
Function:
Regulate cell fate decision for various cell developments, including myogenesis, neurogenesis, and lymphocyte development.
Ligand Receptor:
Notch molecules
Antigen References:
1. Moriyama, Y. et al. 2008. Int J Immunol 20:763 2. Ehebauer, M.T. et al. 2006. Biochem J 392:13 3. Shimizu, K. et al. 2000. Mol Cell Biology 20:18 4. Hellstrom, M. et al. 2007. Nature 445:776 5. Luo, B. et al. 1997. Mol Cell Biol. 17:6057 6. Stanley, P. et al. 2009. Immunol Rev. 230(1):201
