The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC - Quality tested IHC (frozen)*, IP*, activation* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications include: immunoprecipitation and stimulation of TNF-a production by monocytes
Application References:
1. Aguilar, H., et al. 2004. J. Immunol. 173: 6703
Human peripheral blood monocytes stained with UP-H2 PE
Description:
IREM-2 (immune receptor expressed by myeloid cells 2), also known as CMRF35-A5, LMIR6, CD300Le or CD300e, is a 34 kDa type I single transmembrane glycoprotein. It is a member of the CD300 family and of the Ig-superfamily. IREM-2 is expressed on monocyte and myeloid dendritic cells, but not on granulocytes and lymphoid dendritic cells. IREM-2 is an activating receptor through interaction with DAP12.
Other Names:
CD300e, CMRF35-A5, LMIR6,
Structure:
34 kD, type I transmembrane glycoprotein, CD300 family, Ig superfamily
Distribution:
Monocytes, macrophages, dendritic cells
Function:
Activation of myeloid cells
Antigen References:
1. Aguilar, H., et al. 2004. J. Immunol. 173: 6703