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The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported application (for the relevant formats) include: immunofluorescence microscopy1, immunopricipitation2, and blocking3.
This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.
Application References:
1. Lafuste P, et al. 2005. Mol. Biol. Cell. 16:861. (IF) 2. Chen C, et al. 2006. Immunity 25:895. (FC, IP) 3. Taooka Y, et al. 1999. J. Cell Biol. 145:413. (Block)
Human peripheral blood granulocytes were stained with integrin α9β1 (clone Y9A2) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
Integrin α9β1 is a transmembrane protein belonging to the integrin family. It is widely expressed in airway epithelium, different types of muscle cells, and hepatocytes. In the hematopoietic system, integrin α9β1 is highly expressed on human neutrophils and at low level on monocytes. Multiple ligands have been identified for this integrin including the inducible endothelial counter-receptor, vascular cell adhesion molecule-1 (VCAM-1), the extracellular matrix proteins tenascin C and osteopontin, some members of ADAMs family, coagulation factor XIII, and von Willebrand factor. It has been reported that binding of the α9 cytoplasmic domain to spermidine/spermine N(1)-acetyltransferase (SSAT) mediates α9β1 enhancement of cell migration, mediates adhesion to the extracellular matrix proteins, and is involved in G-CSF signaling.Morerecently, integrin α9β1 was found on hematopoietic stem and progenitor cells to mediate stem cells adhesion, proliferation, and differentiation.
Structure:
Heterodimeric proteins made up of α9 and β1 subunits
Distribution:
Neutrophil, monocytes, endothelial cells, hepatopoietic cells, and different type of muscle cells
Function:
Receptor for VCAM-1, involved in cell adhesion and migration
Ligand Receptor:
VCAM-1, ECM proteins tenascin C, osteopontin
Interaction:
Recognizes α9 integrin expressed as a heterodimer in association with the β1 integrin.
Cellular Targets:
Binds VCAM-1
Antigen References:
1. Schreiber TD, et al. 2009 Haematologica 94:1495. 2. Chen C, et al. 2006 Immunity 25:895. 3. Taooka Y, et al. 1999 J. Cell Biol. 145:413. 4. Palmer EL, et al. 1993 J. Cell Biol. 123:1289.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human Integrin α9β1
Human peripheral blood granulocytes were stained with purified integrin α9β1 (clone Y9A2) (filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG-PE.
LEAF™ Purified anti-human Integrin α9β1
Human peripheral blood granulocytes were stained with integrin α9β1 (clone Y9A2) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
PE anti-human Integrin α9β1
Human peripheral blood granulocytes were stained with integrin α9β1 (clone Y9A2) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
