Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
test sizes: lot-specific; µg size: 0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
ELISA Capture2: The purified MH9A4 antibody is useful as the capture antibody in a human IL-9 sandwich ELISA assay, when used in conjunction with the biotinylated MH9D1 (Cat. No. 507702) antibody as the detecting antibody. Flow Cytometry: The fluorochrome-labeled MH9A4 antibody is useful for intercellular immunofluorescent staining and flow cytometric analysis to identify human IL-9-producing cells in mixed cell populations. View intracellular cytokine staining protocol
Application References:
1. Jenmalm M, et al. 2001. Clin. Exptl. Aller. 31:1528. 2. Faulkner H, et al. 2002. J. Infec. Diseas. 185:665. 3. Chang HC, et al. 2010. Nat. Immunol. 11:527. (ELISA) PubMed
Enriched human CD4+ T cells were stimulated with PMA+ionomycin, then intracellular stained with anti-IFN-γ (4S.B3) FITC and MH9A4 PE
IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 has also been reported to protect human T cells from apoptosis induced by IL-2 withdrawal. IL-9 is upregulated in human eosinophils by TNF-α and IL1-β. IL-9 has been reported to downregulate the oxidative burst in activated human alveolar macrophages and induce TGF-β production. The recently characterized Th-9 helper cell lineage is characterized by the production of IL-9. The MH9A4 antibody reacts with human IL-9. The MH9A4 antibody can neutralize the bioactivity of natural or recombinant IL-9.
Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
Antigen References:
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Quesniaux V. 1992. Research Immunology 143:385. 3. Renauld J, et al. 1993. Adv. Immunol. 54:79. 4. Yang Y. 1992. Leuk. Lymphoma 8:441.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human IL-9
PE anti-human IL-9
Enriched human CD4+ T cells were stimulated with PMA+ionomycin, then intracellular stained with anti-IFN-γ (4S.B3) FITC and MH9A4 PE
Alexa Fluor® 647 anti-human IL-9
PMA/ionomycin-stimulated TH2 polarized lymphocytes intracellularly stained with anti-CD3 (UCHT1) PE and MH9A4 Alexa Fluor® 647
