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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
ELISA or ELISPOT Capture1-3,8: The purified MQ2-13A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MQ2-39C3 antibody (Cat. No. 501202/501203) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. Flow Cytometry4: The fluorochrome-labeled MQ2-13A5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-6 -producing cells within mixed cell populations. View intracellular cytokine staining protocol Neutralization1-3,6,7: The LEAF™ purified antibody (Endotoxin Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining5 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry. Note: For testing human IL-6 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430501 to 430506) are specially developed and recommended.
Application References:
1. Abrams, J., et al. 1992. Immunol. Rev. 127:5. 2. Abrams, J. 1995. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.20. 3. Gaines Das, R., et al. 1993. J. Immunol. Meth. 160:147. 4. Enriquez, J., et al. 2002. Adv. Perit Dial. 18:177. 5. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. 6. Zou, J-p., et al. 1999. J. Immunol. 162:4882. 7. Wyant, T. L., et al. 1999. Infect. Immun. 67:1338. 8. Lesmeister, M. J., et al. 2005. Reproductive Biology and Endocrinology 3:74. 9. Zhang, H., et al. 2011. PNAS. 108:13456. PubMed.
LPS-stimulated (6 hours) human peripheral blood monocytes were surface stained with CD14 FITC and then intracellular stained with MQ2-13A5 PE
IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes. The MQ2-13A5 antibody can neutralize the bioactivity of natural or recombinant IL-6.
Other Names:
Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
Structure:
Neuropoietic cytokine family; 26 kD (Mammalian)
Regulation:
Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
Cellular Sources:
T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Cellular Targets:
T cells, B cells, hepatocytes, cholinergic neurons
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Hirano, T. 1998. Intl. Rev. Immunol. 16:249. 3. Patterson, P. 1992. Curr. Opin. Neurobiol. 2:94. 4. Van Oers, M., et al. 1993. Ann. Hematology 66:219.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human IL-6
LPS-stimulated (6 hours) human peripheral blood monocytes surface stained with CD14 APC and intracellular stained with MQ2-13A5 FITC
LEAF™ Purified anti-human IL-6
PE anti-human IL-6
LPS-stimulated (6 hours) human peripheral blood monocytes were surface stained with CD14 FITC and then intracellular stained with MQ2-13A5 PE
Purified anti-human IL-6
APC anti-human IL-6
LPS-stimulated (6 hours) human peripheral blood monocytes surface stained with CD14 PE/Cy7 and intracellular stained with MQ2-13A5 APC
