Antibodies
Login
Search   
PE anti-human IL-21 Antibody
PE anti-human IL-21 Antibody
516703 25 tests $140.00     
516704 100 tests $280.00     
Product Details
Clone: 4BG1
Isotype: Mouse IgG2b, κ
Isotype Control:PE Mouse IgG2b, κ Isotype Ctrl
Reactivity: Human
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Enter Lot#:   
Application
References:

1. Rohman M, et al. 2007. FEBS Lett. 581:4001.
2. Onoda T, et al. 2007. Int. Immunol. 19:1191.
PMA+ionomycin-stimulated (6 hours) human peripheral
PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes were surface stained with CD3 APC, then intracellularly stained with 4BG1 PE


Compare all formats



Description:

Interleukin 21 (IL-21) is a potent immunomodulatory cytokine mainly produced by NKT and CD4+ T-cells, particularly the inflammatory Th17 subset and has pleiotropic effects on both innate and adaptive immune responses.  These actions include positive effects such as enhancing proliferation of NK cells and cytotoxic T cells, and inhibitory effects on the antigen-presenting function of dendritic cells.  It can also be proapoptotic for B cells and NK cells.  Recent studies have shown that IL-21 is also an autocrine cytokine that potently induces Th17 differentiation and suppresses Foxp3 expression, and serves as a target for treating inflammatory diseases.
Other Names: Interleukin 21
Distribution: Th17
Function: Immunomodulation
Cellular Sources: NK-T cells and T cells
Cellular Targets: cytotoxic T cells (CTL) and Natural Killer (NK) cells, that can destroy malignant or infected cells
Receptors: IL-21R
Antigen
References:

1. Nurieva R. 2007. Nature 448:416.
2. Parrish-Novak J, et al. 2002. J. Leukocyte Biol. 72:856.
3. Dumoutier L, et al. 2000. Proc. Natl. Acad. Sci. USA 97:10144.
4. Asao H, et al. 2001. J. Immunol. 167:1.
5. Parrish-Novak J, et al. 2000. Nature 408:57.
GeneID: 59067
Latest Publications: View the latest IL-21 articles on HighwirePress.com
UniProt: View information about IL-21 on UniProt.org
Keywords: PE anti-human IL-21, 4BG1, PE, Interleukin 21, Human, Immunology, Antibodies
Related Products
DescriptionCloneApplications
PE Mouse IgG2b, κ Isotype CtrlMPC-11FC, ICFC
Cell Staining BufferFC, ICC, ICFC
RBC Lysis Buffer (10X)FC, ICFC
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
x
Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • PE anti-human IL-21
    PMA+ionomycin-stimulated (6 hours) human peripheral

    PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes were surface stained with CD3 APC, then intracellularly stained with 4BG1 PE

x
Copyright © 2011 BioLegend, Inc. All rights reserved.