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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
ELISA or ELISPOT Capture1,2: The purified JES10-5A2 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5020 antibody (Cat. No. 502001) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. Flow Cytometry3,7: The fluorochrome-labeled JES10-5A2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-13 -producing cells within mixed cell populations. View intracellular cytokine staining protocol Neutralization1: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for neutralization of human IL-13 bioactivity (Cat. No. 501910). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining4-6,8 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.
Application References:
1. McKenzie, A., et al. 1994. Curr. Prot. Immunol.. John Wiley & Sons. New York. Section 6.18. 2. Abrams, J. 1995. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.20. 3. Schaerli, P., et al. 2000. J. Exp. Med. 192:1553. 4. Andersson, J., et al. 1994. Immunology 83:16. 5. Skansen-Saphir, U., et al. 1994. Eur. J. Immunol. 24:916. 6. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. 7. Gelfand, E. W., et al. 2006. J. Aller. and Clinic. Immunol. 117:577. 8. Knorr, C., et al. 1999. Am. J. Pathol. 155:2019.
PMA+ionomycin-stimulated T lymphocytes intracellularly stained with JES10-5A2 PE and CD4 (RPA-T4) APC
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 lymphocytes. IL-13 shares 30% amino acid sequence homology with IL-4 and demonstrates similar biological activities. The biological activities of IL-13 include: suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of proinflammatory cytokine secretion. The JES10-5A2 antibody reacts with human interleukin-13 (IL-13). The JES10-5A2 antibody can neutralize the bioactivity of natural or recombinant IL-13.
Other Names:
Interleukin-13, NC30
Structure:
Cytokine; 17 kD (Mammalian)
Regulation:
IL-13Rα2 possible antagonist
Cellular Sources:
Activated T cells, mast cells, NK cells
Cellular Targets:
B cells, monocytes, macrophages, basophils
Receptors:
Moderate affinity IL-13Rα1; high affinity heterodimer IL-13Rα1/IL-4Rα also binds IL-4; both receptor complexes have γ-subunit in common with IL-2R, IL-4R, IL-7R, IL-15R ; high affinity IL-13Rα2 incapable of signaling
Bioactivity/Activities:
Suppression of macrophage cytotoxic activity; upregulation of IL-1RA expression; suppression of IL-1, IL-6, IL-8, IL-10, IL-12, chemokines MCP, MIP-1; differentiation of B cells, monocytes
Antigen References:
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Hilton, D., et al. 1996. P. Natl. Acad. Sci. USA 93:497. 3. Obiri, N., et al. 1995. J. Biol. Chem. 270:8797. 4. Ochensberger, B., et al. 1996. Blood 88:3028.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human IL-13
Enriched human CD4+ T cells were stimulated with PMA+ionomycin, then surface stained with CD4 PE/Cy5 and intracellular stained with APC rat IgG1,k isotype control (top) or APC JES10-5A2 (bottom)
LEAF™ Purified anti-human IL-13
PE anti-human IL-13
PMA+ionomycin-stimulated T lymphocytes intracellularly stained with JES10-5A2 PE and CD4 (RPA-T4) APC
Purified anti-human IL-13
PerCP/Cy5.5 anti-human IL-13
PMA-restimulated Th2 polarized human peripheral blood lymphocytes intracellular stained with CD3 FITC and JES10-5A2 PerCP/Cy5.5
