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PE anti-human Ig light chain κ Antibody
PE anti-human Ig light chain κ Antibody
316507 25 tests ¥18,000     
316508 100 tests ¥40,000     
Product Details
Clone: MHK-49
Isotype: Mouse IgG1, κ
Isotype Control:PE Mouse IgG1, κ Isotype Ctrl (FC)
Reactivity: Human
Immunogen: human IgGs and IgM, IgE, IgA cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC, ICFC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Enter Lot#:   
 CD19<sup>+</sup> human B-lymphocytes stained with
CD19+ human B-lymphocytes stained with MHL-38 (anti-λ) FITC and MHK-49 (anti-κ) PE


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See PE spectral data



Description: The MHK-49 antibody reacts with both soluble and membrane human immunoglobulin light chain kappa (k). It does not react with human immunoglobulin light chain lambda (λ) or heavy chain (μ). The MHK-49 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Other Names: Immunoglobulin light chain kappa
Structure: Ig family
Distribution: B cells
GeneID: 3514
Latest Publications: View the latest kappa light chain articles on HighwirePress.com
UniProt: View information about kappa light chain on UniProt.org
Keywords: PE anti-human Ig light chain κ, MHK-49, PE, Immunoglobulin light chain kappa, Human, Immunology, Antibodies
Related Products
DescriptionCloneApplications
Cell Staining BufferFC, ICC, ICFC
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Human TruStain FcX™ (Fc Receptor Blocking Solution)FC, ICC, ICFC
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-human Ig light chain κ
    Over night cultured human peripheral

    Over night cultured human peripheral blood lymphocytes stained with purified MHK-49, followed by anti-mouse IgG FITC

  • Biotin anti-human Ig light chain κ
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with biotinylated MHK-49, followed by Sav-PE

  • FITC anti-human Ig light chain κ
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with MHK-49 FITC

  • PE anti-human Ig light chain κ
    CD19<sup>+</sup> human B-lymphocytes stained with

    CD19+ human B-lymphocytes stained with MHL-38 (anti-λ) FITC and MHK-49 (anti-κ) PE

  • APC anti-human Ig light chain κ
    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells stained with MHK-49 APC and anti-CD19 (HIB19) PE

  • Alexa Fluor® 647 anti-human Ig light chain κ
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with MHK-49 Alexa Fluor® 647

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