The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Lee, N., et al. 1998. Proc. Natl. Acad. Sci. USA. 95: 5199 2. Wooden, SL., et al. 2005. J. Immunol. 175: 1383 3. Monaco, EL., et al. 2008. J. Immunol. 181: 5442
Human peripheral blood lymphocytes stained with 3D12 PE
Description:
HLA-E is a non-classical MHC class I (MHC-Ib) molecule. It is characterized by limited polymorphism and ubiquitous expression. HLA-E, as other MHC-I molecules, is heterodimerized with β2-microglobulin. HLA-E interacts with CD94/NKG2 receptor to regulate NK cell cytolysis activities.
Other Names:
HLA class I histocompatibility antigen, alpha chain E, HLAE
Structure:
HLA family, Ig superfamily, 42 kDa
Distribution:
Broadly expressed
Function:
Mediate cell recognition by NK cells
Ligand Receptor:
CD94/NKG2
Antigen References:
1. Sullivan, LC., et al. 2008. Tissue Antigen. 72: 415 2. Koller, BH., et al. 1988. J. Immunol. 141: 897
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.