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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: The DJR3 antibody is useful for immunofluorescent staining and flow cytometric analysis of DcR1/TRAIL-R3 receptor expression. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 307006) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 307004) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
Application References:
1. Uno K, et al. 2003. Blood 101:3658. 2. Sato K, et al. 2005. J. Immunol. 174:4025.
Human peripheral blood granulocytes stained with DJR3 PE
DcR1 is a 65 kD GPI-anchored member of the TNFR superfamily (TNFRSF10C) also known as TRAIL-R3, TRID, CD263, and LIT. Although not expressed by most tumors, DcR1 is expressed at low levels on normal tissues, acting as a TRAIL decoy receptor, thus inhibiting apoptosis. DcR1 is termed a decoy receptor, as it does not have a functional death domain to induce apoptosis following TRAIL ligation.
Other Names:
TRAIL-R3, TRID, LIT, CD263, TNFRSF10C
Structure:
TNFR superfamily, GPI-anchored, truncated death domain, 65 kD
Distribution:
Most normal tissues, not tumors
Function:
TRAIL decoy, inhibits apoptosis
Ligand Receptor:
TRAIL
Antigen References:
1. Schneider P, et al. 1997. J. Biol. Chem. 272:25417. 2. Pan G, et al. 1997. Science 277:815.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-human DcR1 (TRAIL-R3, CD263)
Human peripheral blood granulocytes stained with DJR3 PE
Purified anti-human DcR1 (TRAIL-R3, CD263)
Human peripheral blood granulocytes stained with purified DJR3, followed by biotinylated anti-mouse IgG and Sav-PE
