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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application References:
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Human peripheral blood platelets stained with HI9a PE
CD9 is a 24 kD type III transmembrane protein also known as tetraspanin, MRP-1 and DRAP-24. It is a member of the tetraspan family (spanning the membrane four times) found on platelets, B cell progenitors, activated lymphocytes, granulocytes, endothelial cells and epithelial cells. CD9 induces adhesion, platelet aggregation, and B cell development. CD9 has been shown to associate with CD63, CD81, CD82, and CD36 and to bind to β1 integrins.
Other Names:
Tetraspanin, MRP-1, DRAP-24
Structure:
Tetraspan family, type III transmembrane protein, 24 kD
Distribution:
Platelets, B cell progenitors, activated lymphocytes, granulocytes, endothelial and epithelial cells
Function:
Adhesion, platelet activation, B cell development
Ligand Receptor:
Associates with CD63, CD81, CD82 and CD36, binds PSG17
Antigen References:
1. Miao WM, et al. 2001 Blood 97:1689. 2. Ellerman DA, et al. 2003 Mol. Biol Cell. (Epub ahead of print). 3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD9
Human platelets stained with purified HI9a, then detected with anti-mouse IgGs FITC
FITC anti-human CD9
Human peripheral blood platelets stained with HI9a FITC
PE anti-human CD9
Human peripheral blood platelets stained with HI9a PE
