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The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: Western Blot2 and immunoprecipitation3.
Application References:
1. Menno C, et al. 2010. J. Clin. Invest. 4:1265. 2. Oren R, et al. 1990. Mol. Cell. Biol. 8:4007. (WB) 3. Clark K, et al. 2004. J. Biol. Chem. 10:1074. (IP) 4. Mochida K, et al. 2008. J. Virol. 13:6711.
Human peripheral blood lymphocytes stained with CD81 (5A6) PE
CD81 is a 26 kD non-glycosylated member of the tetraspanin superfamily (TM4SF), also known as TAPA-1 (target of an antiproliferative antibody). CD81 is expressed on T and B cells, NK cells, monocytes, dendritic cells, thymocytes, endothelial cells, and fibroblasts. It also has low levels of expression on granulocytes. CD81 induces B cell adhesion via VLA-4 integrin and has been shown to play a role in early T cell development. CD81 associates with several other cell-surface proteins in a multimolecular complex, including CD19, CD21, CD20, CD37, CD53, and CD82 in B cells, and CD4, CD8, and CD82 in T cells.
Structure:
26 kD, type III transmembrane protein, member of the TM4SF tetraspanin family. Complexed with CD82, CD19, CD21, or CD4, CD8.
Distribution:
T, NK, monocytes, B cells, endothelial and epithelial cells, low on granulocytes
Function:
Regulate cell activation and growth, cell aggregation
Antigen References:
1. Menno C, et al. 2010. J. Clin. Invest. 4:1265. 2. Fearon D, et al. 1995. Annu. Rev. Immunol. 13:127. 3. Wright M, et al. 1994. Immunol. Today 15:588.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD81 (TAPA-1)
Human peripheral blood lymphocytes stained with purified CD81 (5A6) conjugated with PE
FITC anti-human CD81 (TAPA-1)
Human peripheral blood lymphocytes stained with CD81 (5A6) FITC
PE anti-human CD81 (TAPA-1)
Human peripheral blood lymphocytes stained with CD81 (5A6) PE
