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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Human colorectal adenocarcinoma cell line HT29 stained with ASL-32 PE
The ASL-32 mAb reacts with an antigen epitope shared by CD66a, c and e. CD66a/c/e are members of the CEA (carcinoembryonic antigen) family of the Ig superfamily. CD66 plays a role in hemophilic and heterophilic adhesion. CD66a, also known as CEACAM1 and BGP (biliary glycoprotein), is mainly expressed on granulocytes, binds to CD66a, c, e, and CD62E. CD66c (known as CEACAM6, NCA) is expressed on granulocytes and epithelial cells. The ligands of CD66c are CD66a-e, CD62E and Galectins. CD66e molecule is also called as CEA or CEACAM5, primarily found on epithelial cells. CD66e binds to CD66a, c and e.
Other Names:
CEACAM1/5/6, BGP, CEA, NCA
Structure:
Member of the CEA (carcinoembryonic antigen) family of the Ig superfamily
Distribution:
Granulocytes, epithelial cells
Function:
Homophilic and heterophilic adhesion
Ligand Receptor:
CD66a-e, CD62E, galectins
Antigen References:
1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss. A John Wiley & Sons Inc. Publication
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT29 stained with purified ASL-32 conjugated to PE
PE anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT29 stained with ASL-32 PE
Alexa Fluor® 488 anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT-29 stained with ASL-32 Alexa Fluor® 488
