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PE anti-human CD43 Antibody
PE anti-human CD43 Antibody
343203 25 tests $80.00 Qty:
    
343204 100 tests $185.00 Qty:
    

Product Details

 
Clone: CD43-10G7 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:PE Mouse IgG1, κ Isotype Ctrl
Reactivity: Human
Immunogen: MV4-11 cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.

Excitation
Laser:
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
COA:
Enter Lot#:   
Application
References:

  Publication Library
PE anti-human CD43 Antibody CD43-10G7 Image 1
Human peripheral blood lymphocytes stained with CD43-10G7 PE and CD3 APC (top) or mouse IgG1, κ PE isotype control and CD3 APC (bottom).

PE anti-human CD43 Antibody CD43-10G7 Image 2


Compare all formats

See PE spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

CD43, also known as Ly-48, leukosialin, sialophorin, leukocyte sialoglycoprotein, and W3/13, is a large single chain, type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors.

Other Names: Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein
Structure: Single chain, type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 95-130 kD.
Distribution: Most hematopoietic cells, including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, activated B cells, and hemotopoietic progenitor cells.
Function: Plays dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, inducing apoptosis of T cells and hematopoietic progenitors.
Ligand Receptor: CD54, Siglec-1
Antigen
References:

1. Neil Barclay A, et al. 1997. The Leucocyte Antigen Facts Book:Second Edition. Page 238
2. Killeen A, et al. 1987. EMBO J. 6:4029.
3. Thurman EC, et al. 1998. Intl. Immunol. 10:691.
4. Onami TM, et al. 2002. J. Immunol. 168:6022.
5. Tong J, et al. 2004. J. Exp. Med. 199:1277.
6. Jones AT, et al. 1994. J. Immunol. 153:3426.

GeneID: 6693
Latest Publications: View the latest CD43 articles on HighwirePress.com
UniProt: View information about CD43 on UniProt.org
Keywords: PE anti-human CD43, CD43-10G7, PE, Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, Human, Flow Cytometry, Immunology, Antibodies


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Other Formats

DescriptionCloneApplications
APC anti-human CD43CD43-10G7FC
PE/Cy7 anti-human CD43CD43-10G7FC
Purified anti-human CD43CD43-10G7FC, IP


Related Pathways

Pathway
Regulatory T Cells
TH1 Pathway (Cellular Immune Response)
TH2 Pathway (Humoral Immune Response)


Related Categories

Category
Immunology > Human Immunology > NK/NKT > CD43
Immunology > Human Immunology > Monocyte/Macrophage > CD43
Immunology > Human Immunology > CD and Related Molecules > CD43 (Leukosialin) > CD43-10G7
Immunology > Human Immunology > Adhesion Molecules > CD43 > CD43-10G7

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD43
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with CD43-10G7 PE and CD3 APC (top) or mouse IgG1, PE κ isotype control and CD3 APC (bottom).





  • PE anti-human CD43
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with CD43-10G7 PE and CD3 APC (top) or mouse IgG1, κ PE isotype control and CD3 APC (bottom).





  • APC anti-human CD43
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with CD3 (UCHT1) PE and CD43-10G7 APC (top) or mIgG1,k APC isotype control (bottom)

  • PE/Cy7 anti-human CD43
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD43 (clone CD43-10G7) PE/Cy7 or mouse IgG1, κ PE/Cy7 isotype control.

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