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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application References:
1. Croockewit AJ, et al. 1998. Scand. J. Immunol. 47:82. 2. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
Human peripheral blood mononuclear cells stained with 561 PE (lower panel) or PE mouse IgG2a isotype control (upper panel) and CD45 (HI30) PerCP (gated on CD14- cell population)
CD34 is a type I monomeric sialomucin-like glycophophoprotein with the molecular weight about 105-120 kD, also know as gp105-120. It is selectively expressed on majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. The 561 antibody recognized epitope is in class III group which is resistant to sialidase/glycolyprotease and chymopapain treatment. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD34
Human peripheral blood mononuclear cells stained with 561 FITC (lower panel) or FITC mouse IgG2a isotype control (upper panel) and CD45 (HI30) PerCP (gated on CD14- cell population)
FITC anti-human CD34
Human peripheral blood mononuclear cells stained with CD34 (clone 561) FITC (top) or FITC mouse IgG2a isotype control (bottom) and CD45 (clone HI30) PerCP (gated on CD14- cell population).
PE anti-human CD34
Human peripheral blood mononuclear cells stained with 561 PE (lower panel) or PE mouse IgG2a isotype control (upper panel) and CD45 (HI30) PerCP (gated on CD14- cell population)
APC anti-human CD34
Human peripheral blood leukocytes stained with CD45 (HI30) FITC and 561 APC (top) or mIgG1,κ APC isotype control (bottom). Data was generated by gating on live CD14 negative lymphocyte population
