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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
Excitation Laser:
Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm)
COA:
Application Notes:
The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6.
Human peripheral blood mononuclear cells stained with 581 PE (lower panel) or PE mouse IgG1 isotype control (upper panel) and CD45 (HI30) PerCP (gated on CD14- cell population)
CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.
1. Krause DS, et al. 1996. Blood 87:1. 2. Puri KD, et al. 1995. J. Cell Biol. 131:261. 3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD34
Human myeloid cell line KG1a stained with purified 581, followed by anti-mouse IgG FITC
FITC anti-human CD34
Human peripheral blood mononuclear cells stained with 581 FITC (lower panel) or FITC mouse IgG1 isotype control (upper panel) and CD45 (HI30) PerCP (gated on CD14- cell population)
PE anti-human CD34
Human peripheral blood mononuclear cells stained with 581 PE (lower panel) or PE mouse IgG1 isotype control (upper panel) and CD45 (HI30) PerCP (gated on CD14- cell population)
Alexa Fluor® 647 anti-human CD34
Human peripheral blood leukocytes stained with 581 Alexa Fluor® 647 and CD45 (HI30) FITC (top) or mIgG1, κ isotype control Alexa Fluor® 647 and CD45 (HI30) FITC (bottom). Cytograms were gated to display CD14 negative lymphocyte population.
APC anti-human CD34
Human peripheral blood leukocytes stained with 581 APC/CD45 FITC/CD14 PE and 7-AAD then analyzed via gating on total live and CD14 negative lymphocyte population
Pacific Blue™ anti-human CD34
Human peripheral blood leukocytes stained with CD34 Pacific Blue™ (clone 581) or mouse IgG1, κ Pacific Blue™ isotype control and costained with CD45 FITC, CD14 PE and 7-AAD, then analyzed via gating on total live and CD14 negative lymphocyte population.
APC/Cy7 anti-human CD34
Human peripheral blood stained with 581 APC/Cy7 or mouse IgG1,k isotype control APC/Cy7. Cytograms were gated to display total live CD14 negative mononuclear cells.
PE/Cy7 anti-human CD34
Human peripheral blood stained with 581 PE/Cy7 or mouse IgG1,k isotype control PE/Cy7. Cytograms were gated to display total live CD14 negative mononuclear cells.
Alexa Fluor® 488 anti-human CD34
Human peripheral blood leukocytes stained with CD45 (HI30) APC and 581 Alexa Fluor® 488 (top) or mIgG1, κ Alexa Fluor® 488 isotype control (bottom). Data was generated by gating on live CD14 negative lymphocyte population.
PerCP anti-human CD34
Human peripheral blood leukocytes stained with CD45 FITC and 581 PerCP (top) or mouse IgG1, κ PerCP isotype control (bottom). Cytogram gated for total live CD14 negative lymphocyte population
PerCP/Cy5.5 anti-human CD34
Human peripheral blood leukocytes stained with CD45 FITC and 581 PerCP/Cy5.5 (top) or mouse IgG1, κ PerCP/Cy5.5 isotype control (bottom). Cytogram gated for total live CD14 negative lymphocyte population
Biotin anti-human CD34
Human peripheral blood lymphocytes stained with biotinylated CD34 (clone 581) (top), or mouse IgG1 isotype control (bottom), followed by Sav-PE and co-stained with CD14 APC and CD45 PerCP. Gated on CD14 dim population.
Alexa Fluor® 700 anti-human CD34
Human peripheral blood mononuclear cells were stained with CD14 PE, CD45 FITC, and CD34 (clone 581) Alexa Fluor® 700 (top) or mouse IgG1, κ Alexa Fluor® 700 isotype control (bottom). Data shown was gated on CD14-negative cell population.
Brilliant Violet 510™ anti-human CD34
Human peripheral blood mononuclear cells were stained with CD45 FITC and CD34 Brilliant Violet 510™ (clone 581) or mouse IgG1, κ Brilliant Violet 510™ isotype control. Data was generated by gating on live CD14 negative cell population.
