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The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
The mAb 8C11 recognizes the amino acids 92–593 of CD325, located between the extracellular cadherin structural domain (EC) 3 and 4. Additional reported applications (for the relevant formats) include: immunofluorescence1,3,6, motility inhibition of N-cadherin-expressing cells2, and Western blot2,4.
Application References:
1. Navarro P, et al. 1998. J. Cell Biol. 140:1475. (IF) 2. Kim JB. 2000. J. Cell Biol. 151:1193. (Block, WB) 3. Puch S, et al. 2001. J. Cell. Sci. 114:1567. (IF) 4. Wahl JK. 3rd, et al. 2003. J. Biol. Chem. 278:17269. (WB) 5. Wein F, et al. 2010. Stem. Cell Res. 4:129. (FC) 6. Jaggi M, et al. 2002. Cell. Commun. Adhes. 9:103. (IF)
Human acute myeloid leukemia cell line (KG1) were stained with CD325 (clone 8C11) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
CD325 (N-cadherin) is a 130 kD, single pass transmembrane protein. Its extracellular region consists of five EC domains and has one cytoplasmic domain. N-cadherin is involved in organogenesis and maintenance of organ architecture by contributing to the sorting of heterogeneous cell types and in the cell adhesion needed to form tissues. N-cadherin is expressed by stem cells, myeloblasts, endothelial cells, and fibroblasts, and also is expressed in neural and muscle tissues and some types of carcinoma cells. CD325 associates with the cytoskeleton trough catenin proteins.
Other Names:
Cadherin-2, Neural cadherin
Structure:
Member of cadherin family, Single pass transmembrane protein, extracellular region consist of five EC domains, one cytoplasmic domain, 130 kD
Distribution:
Stem cells, myeloblasts, endothelial cells, fibroblasts, neural and muscle tissues, some types of carcinoma cells
Function:
Embryonic development, maintain tissue architecture, cell sorting and cell adhesion in tissues, promote motility
Interaction:
Catenins
Antigen References:
1. Colomiere M, et al. 2009. Brit. J. Cancer 100:134. 2. Yan W, et al. 2010. J. Biol. Chem. 285:14042. 3. Mosnier JF, et al. 2009. Mod. Pathol. 22:182. 4. Gao L, et al. 2010. Stem Cells 28:564.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-human CD325 (N-Cadherin)
Human acute myeloid leukemia cell line (KG1) were stained with CD325 (clone 8C11) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
LEAF™ Purified anti-human CD325 (N-Cadherin)
Human acute myeloid leukemia cell line (KG1) stained with CD325 (clone 8C11) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram)
Purified anti-human CD325 (N-Cadherin)
Human acute myeloid leukemia cell line (KG1) stained with CD325 (clone 8C11) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram)
APC anti-human CD325 (N-Cadherin)
Human acute myeloid leukemia cell line (KG1) was stained with CD325 (clone 8C11) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
