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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of acetone-fixed frozen tissue sections.
Application References:
1. Kishimoto T, et al. 1997. Leucocyte Typing VI Garland Press. London. 2. Lerino F, et al. 1993. J. Immunol. 150:1794. 3. Personal communication. 4. van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. 5. Smeltz RB, 2007. J. Immunol. 178:4786.
Human peripheral blood lymphocytes stained with FUN-2 PE
CD32 is a 40 kD polymorphic transmembrane glycoprotein also known as FcγRII and FCRII. It is an immunoglobulin superfamily member expressed on monocytes/macrophages, granulocytes, platelets and B cells. There are at least 6 isoforms of CD32 resulting from alternative mRNA splicing. CD32 mediates phagocytosis and oxidative burst in granulocytes, as well as platelet aggregation and immunomodulation. The extracellular domain of CD32 binds to polymeric and aggregated IgG and immune complexes, while the intracellular domain has been reported to associate with SHP-1 (B1 isoform).
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD32
Human peripheral blood lymphocytes stained with FUN-2 APC
FITC anti-human CD32
Human peripheral blood lymphocytes stained with FUN-2 FITC
PE anti-human CD32
Human peripheral blood lymphocytes stained with FUN-2 PE
Purified anti-human CD32
Human peripheral blood lymphocytes stained with purified FUN-2, followed by anti-mouse IgGs FITC
Alexa Fluor® 647 anti-human CD32
Human peripheral blood lymphocytes stained with FUN-2 Alexa Fluor® 647
