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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7, immunoprecipitation1, activation of T cells2,3,5, and Western blotting9. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 300414).
Application References:
1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP) 2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ) 3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ) 4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC) 5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ) 6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC) 7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC) 8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed 9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB) 10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Human peripheral blood lymphocytes stained
with UCHT1 PE
CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
Other Names:
T3, CD3ε
Structure:
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution:
Mature T and NK-T cells, thymocyte differentiation
Function:
Antigen recognition, signal transduction, T cell activation
Ligand Receptor:
Peptide antigen bound to MHC
Antigen References:
1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD3
Human peripheral blood lymphocytes stained with UCHT1 APC
Biotin anti-human CD3
Human peripheral blood lymphocytes stained with biotinylated UCHT1 and then detected with Sav-PE
FITC anti-human CD3
Human peripheral blood lymphocytes stained with UCHT1 FITC
LEAF™ Purified anti-human CD3
Human peripheral blood lymphocytes stained with LEAF™ purified UCHT1 and then detected with anti-mouse IgGs FITC
PE anti-human CD3
Human peripheral blood lymphocytes stained
with UCHT1 PE
PE/Cy5 anti-human CD3
Human peripheral blood lymphocytes stained with UCHT1 PE/Cy5
Purified anti-human CD3
Human peripheral blood lymphocytes stained with purified UCHT1 and then detected with anti-mouse IgGs FITC
Alexa Fluor® 647 anti-human CD3
Human peripheral blood lymphocytes stained with UCHT1 Alexa Fluor 647®
Alexa Fluor® 488 anti-human CD3
Human peripheral blood lymphocytes stained with UCHT1 Alexa Fluor 488®
Pacific Blue™ anti-human CD3
Human peripheral blood lymphocytes stained with UCHT1 Pacific Blue™
PE/Cy7 anti-human CD3
Human peripheral blood lymphocytes stained with UCHT1 PE/Cy7 and overlayed with isotype control
Alexa Fluor® 700 anti-human CD3
Human peripheral blood lymphocytes stained with UCHT1 Alexa Fluor® 700
APC/Cy7 anti-human CD3
Human peripheral lymphocytes stained with UCHT1 APC/Cy7
PerCP anti-human CD3
Human peripheral blood lymphocytes stained with UCHT1 PerCP
PerCP/Cy5.5 anti-human CD3
Human peripheral blood lymphocytes stained with UCHT1 PerCP/Cy5.5
Brilliant Violet 421™ anti-human CD3
Human peripheral blood lymphocytes were stained with CD3 (clone UCHT1) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Brilliant Violet 570™ anti-human CD3
Human peripheral blood lymphocytes were stained with CD19 APC and CD3 (clone UCHT1) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom).
