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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application References:
1. Compte E, et al. 2004. Eur. J. Immunol. 34:2089
Human peripheral blood lymphocytes stained with BT3.1 PE and anti-CD3 (HIT3a) APC
CD277 (known as BT3.1, BTN3A1, BTF5) is a 56 kD type I transmembrane glycoprotein. It is one of the three (BT3.1, BT3.2, and BT3.3) BT3 subfamily members. BT3 belongs to butyrophilin (BT)/B7 family and Ig superfamily. BT3.1 is expressed by T cells, B cells, monocytes, NK cells, dendritic cells, subset of stem cells, endothelial cells and some tumor cells. It has been proposed to regulate T cell activation through interaction with its counter-receptor (BT3.1-R).
Other Names:
BT3.1, BTN3A1, BTF5, Butyrophilin subfamily 3 member A1
Structure:
Butyrophilin/B7 family, Ig superfamily
Distribution:
T cells, B cells, monocytes, NK cells, dendritic cells, subset of stem cells, endothelial cells and some tumor cells
Function:
T cell activation
Ligand Receptor:
BT3.1-R
Antigen References:
1. Tazi-Ahnini R, et al. 1997. Immunogenetics. 47:5 2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD277 (BT3.1)
Human peripheral blood lymphocytes stained with purified BT3.1 conjugated to PE and anti-CD3 (HIT3a) APC
PE anti-human CD277 (BT3.1)
Human peripheral blood lymphocytes stained with BT3.1 PE and anti-CD3 (HIT3a) APC
