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The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
Excitation Laser:
Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm)
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: blocking the efflux of fluorescent dyes1, 2, immunoprecipitation1, and immunohistochemical staining of tissue sections of squamous cell carcinoma3.
CD243 (MDR-1) belongs to the ATP binding cassette (ABC) transporter family. With an approximate molecular mass of 170 kD, it consists of two homologous halves. Each half contains two hydrophobic transmembrane domains (TMDs) and two hydrophilic nucleotide binding domains (NBDs). The TMDs span the membrane six times, forming a chamber with a 12 transmembrane α-helix structure. NBDs drive the transport process through ATP coupling and hydrolysis and are located at the cytoplasmic face of the membrane. CD243 transports various molecules across cellular membranes and is involved in multidrug resistance. MDR-1 is expressed on hematopoietic stem cells, T cells, B cells, and NK cells as well as on many multidrug resistant neoplastic cells. CD243 interacts with Caveolin, RING finger protein 1B, AAP1, p53, Orphan nuclear receptor PX, and cytochrome P450.
Other Names:
Multidrug resistance protein 1, ATP-binding cassette sub-family B member 1, P-glycoprotein 1
Structure:
Member of ABC transporters family, MW ~170 kD. Consists of two homologous halves, each comprising two hydrophobic transmembrane domains (TMDs) and two hydrophilic nucleotide binding domains (NBDs).
Distribution:
Hematopoietic stem cells, T and B cells, natural killer (NK) cells, and many multidrug resistant neoplastic cells.
Function:
Transport various molecules across cellular membranes. Involved in multidrug resistance.
Cellular Targets:
Caveolin, RING finger protein 1B, AAP1, p53, Orphan nuclear receptor PXR, cytochrome P450.
Antigen References:
1. Dulucq S, et al. 2008. Blood 112:2024. 2. Gottesman MM, et al. 2009. Nat. Biotechnol. 27:546. 3. Andreadis C, et al. 2007. Blood 109:3409.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD243 (MDR-1)
Human neuroblastoma cell line SK-N-FI stained with purified UIC2 conjugated with PE
LEAF™ Purified anti-human CD243 (MDR-1)
Human neuroblastoma cell line SK-N-FI stained with purified UIC2 conjugated with PE
PE anti-human CD243 (MDR-1)
Human neuroblastoma cell line SK-N-FI stained with UIC2 PE
APC anti-human CD243 (MDR-1)
Human neuroblastoma cell line, SK-N-FI, was stained with human CD243 (clone UIC2) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
