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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen tissue sections1, immunofluorescence2
Application References:
1. Matsuzaki H and Seon BK. 1987. Cancer Res. 47:4283. 2. Seon BK, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:845.
Human T lymphoblastic leukemia cell line, Jurkat, stained with SN1a (M3-3D9) PE
CD231 is 150 kD (28-45 kD reduced) cell surface glycoprotein belonging to the Transmembrane 4 Superfamily (TM4SF also known as tetraspanins). It was originally described as T-cell Acute Lymphoblastic Leukemia Antigen (TALLA or TALLA-1). CD231 is expressed on T-cell acute lymphoblastic leukemia, neuroblastoma cells, skeletal muscle, spleen and normal brain neuron. CD231 plays a role in integrin-mediated migration, however, its biological function is not fully clear yet.
T-cell acute lymphoblastic leukemia, skeletal muscle, spleen, neuroblastoma cells and normal brain neuron
Antigen References:
1. Matsuzaki H and Seon BK. 1987. Cancer Res. 47:4283. 2. Seon BK, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:845. 3. Hara H and Seon BK. 1986. Proc. Natl. Acad. Sci. USA 84:3390.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-human CD231 (TALLA)
Human T lymphoblastic leukemia cell line, Jurkat, stained with SN1a (M3-3D9) PE
Purified anti-human CD231 (TALLA)
Human T-lymphoblastic leukemia cell line Jurkat stained with purified SN1a (M3-3D9), followed by anti-mouse IgG FITC
