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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections.
Application References:
1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. Oxford University Press. New York. 2. Clark E. 1993. J. Immunol.. 150:4715. 3. Shan D and O. Press. 1995. J. Immunol.. 154:4466.
Human peripheral blood lymphocytes stained
with HIB22 PE
CD22 is a 130 kD type I transmembrane glycoprotein also known as Siglec-2 and BL-CAM. It is a member of the immunoglobulin superfamily (sialoadhesion subgroup). CD22 is expressed in the cytoplasm of pro-B and pre-B cells, and on the surface of mature B and activated B cells, but not on plasma cells. CD22 is present in the B cell receptor complex and associates with SHP-1, Syk, Lck, Lyn, and phospholipase Cγ1. A primary function of CD22 is thought to be in limiting antigen receptor signaling by modulating B cell activation threshold. CD22 has been shown to bind to CD45RO and CD75, although the natural ligands for this molecule remain controversial.
Other Names:
BL-CAM, Siglec-2, Lyb8
Structure:
Ig superfamily, a type I glycosylated integral transmembrane protein, 120-130 kD
Distribution:
B cells
Function:
Adhesion, B-monocyte and B-T interactions
Ligand Receptor:
CD45RO, CD75
Antigen References:
1. Clark E. 1993. J. Immunol. 150:4715. 2. Shan D, et al. 1995. J. Immunol. 154:4466.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human CD22
Human peripheral blood lymphocytes stained HIB22 FITC
PE anti-human CD22
Human peripheral blood lymphocytes stained
with HIB22 PE
PE/Cy5 anti-human CD22
Human peripheral blood lymphocytes stained HIB22 PE/Cy5
Purified anti-human CD22
Human peripheral blood lymphocytes stained with purified HIB22, followed by anti-mouse IgGs FITC
APC anti-human CD22
Human peripheral blood lymphocytes stained with HIB22 APC
