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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application References:
1. Wood GS, et al. 1983. J. Immunol. 131(1):212
Human T lymphoblastic leukemia cell line Molt-4 stained with SK9 PE
CD1a is a 49 kD member of the immunoglobulin superfamily also known as T6 and R4. It is a type I membrane glycoprotein with structural similarities to MHC class I and is non-covalently associated with β2-microglobulin. CD1a plays a role in non-peptide glycolipid antigen presentation to CD1-restricted T cells. It is expressed on cortical double positive and single positive thymocytes, Langerhans cells, and dendritic cells. In addition to antigen presentation, CD1a has been implicated in thymic T cell development.
Other Names:
T6, R4, Leu-6
Structure:
Ig superfamily, MHC I-like molecule, type I transmembrane glycoprotein, 49 kD
Antigen presentation, lymphocyte activation, thymic T cell development
Ligand Receptor:
CD1-restricted TCRs
Antigen References:
1. Wood GS, et al. 1983. J. Immunol. 131(1):212 2. Wood GS, et al. 1985. Am J Pathol. 120(3):371 3. Martin LH, et al 1987. P. Natl. Acad. Sci. USA 84:9189
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD1a
Human T lymphoblastic leukemia cell line Molt-4 stained with SK9 PE
PE anti-human CD1a
Human T lymphoblastic leukemia cell line Molt-4 stained with SK9 PE
Biotin anti-human CD1a
Human T lymphoblastic cell line, MOLT-4, stained with biotinylated SK9, followed by Sav-PE
