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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1, and ELISA capture1
.
Application References:
1. Pollok-Kopp B, et al. 2003. J. Biol. Chem. 278:2190. (IF ELISA Capture) 2. Huttenrauch F, et al. 2002. J. Biol. Chem. 277:30769. (FC) 3.MondeK,et al.2007.J. Biol Chem. 282:36923.PubMed
Human peripheral blood lymphocytes stained with T21/8 PE
CD195, also known as CCR5, is a 45 kD G protein-coupled seven transmembrane CC-chemokine receptor. It binds to MIP-1α, MIP-1β, and RANTES and is expressed on a subset of T cells and monocytes. CD195 mediates an intracellular signal thought to induce differentiation and proliferation. CCR5 has also been shown to act as a co-receptor for R5 HIV-1 cell entry; modification of CCR5 by sulfation contributes to the efficiency of HIV-1 entry. The T21/8 monoclonal antibody has high affinity to CCR5 N-terminal epitope (Met1-Lys22) and is useful for flow cytometric analysis, immunoprecipitation, immunofluorescence microscopy, and ELISA assay of CCR5 expression.
Other Names:
CD195, CCR5, C-C chemokine receptor type 5, HIV-1 fusion co-receptor
Structure:
G-coupled receptor family 1, membrane protein, 45 kD
Distribution:
Subset of T cells, monocytes
Function:
Binds C-C chemokines and transduces an intracellular signal thought to result in proliferation and differentiation. Acts as a co-receptor with CD4 for HIV-1
Ligand Receptor:
MIP-1a, MIP-1ß, RANTES
Antigen References:
1. Samson M, et al. 1996. Biochemistry 35:3362. 2. Raport CJ, et al. 1996. J. Biol. Chem. 271:17161. 3. Combadiere C, et al. 1996. J. Leukoc. Biol. 60:147. 4. Deng H, et al. 1996. Nature 381:661.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD195 (CCR5)
Human peripheral blood lymphocytes stained with purified T21/8, followed by anti-mouse IgG FITC
Biotin anti-human CD195 (CCR5)
Human peripheral blood lymphocytes stained with biotinylated T21/8, followed by Sav-PE
PE anti-human CD195 (CCR5)
Human peripheral blood lymphocytes stained with T21/8 PE
