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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining2,3 of acetone-fixed frozen tissue sections, immunofluorescence microscopy6, and blocking of CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-14,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 306512).
Application References:
1. McKnight A, et al. 1997. J. Virol. 71:1692. 2. Endres MJ, et al. 1996. Cell 87:745. (IHC) 3. Volin MV, et al. 1998. Biochem. Biophys. Res. Commun. 242:46. (IHC) 4. Berndt C, et al. 1998. P. Natl. Acad. Sci. USA 95:12556. (Block) 5. Ullrich CK, et al. 2000. Blood 96:1438. (Block) 6. Murga M, et al. 2005. Blood 105:1992. (IF) 7. Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) PubMed 8. Isnardi I, et al. 2010. Blood 115:5026. PubMed 9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Human peripheral blood lymphocytes stained
with 12G5 PE
CD184, also known as fusin or CXCR4, is a 45 kD seven transmembrane G-protein-linked CXC chemokine receptor. CD184 is widely expressed on blood and tissue cells, including B and T cells, monocytes, macrophages, dendritic cells, granulocytes, megakaryocytes/platelets, lymphoid, myeloid precursor cells, endothelial cells, epithelial cells, astrocytes, and neurons, among other tissue cells. CD184 is the receptor for CXC chemokine SDF-1, mediates blood cell migration, and is involved in B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, and cerebellar development. CXCR4 is also a coreceptor of X4 HIV-1 and an alternative receptor for some isolates of HIV-2. The 12G5 antibody has been reported to block CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-1.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD184 (CXCR4)
Human peripheral blood lymphocytes stained with 12G5 APC
Biotin anti-human CD184 (CXCR4)
Human peripheral blood lymphocytes stained with biotinylated12G5, followed by Sav-PE
LEAF™ Purified anti-human CD184 (CXCR4)
Human peripheral blood lymphocytes stained with LEAF™ purified 12G5, followed by biotinylated anti-mouse IgG and Sav-PE
PE anti-human CD184 (CXCR4)
Human peripheral blood lymphocytes stained
with 12G5 PE
PE/Cy5 anti-human CD184 (CXCR4)
Human peripheral blood lymphocytes stained with 12G5 PE/Cy5
Purified anti-human CD184 (CXCR4)
Human peripheral blood lymphocytes stained with purified 12G5, followed by biotinylated anti-mouse IgG and Sav-PE
PerCP/Cy5.5 anti-human CD184 (CXCR4)
Human peripheral blood lymphocytes stained with 12G5 PerCP/Cy5.5
PE/Cy7 anti-human CD184 (CXCR4)
Human peripheral blood lymphocytes stained with 12G5 PE/Cy7
Brilliant Violet 421™ anti-human CD184 (CXCR4)
Human peripheral blood lymphocytes were stained with CD184 (clone 12G5) Brilliant Violet 421™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
