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The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1 and immunohistochemistry of frozen and paraffin-embedded samples2.
Application References:
1. Tsyganezawa K, et al. 1998. Blood 92:4317. (IP) 2. Kiyokawa N, et al. 2004. Modern Pathol. 17:423. (ICFC, IHC)
Human cell lines REH (pro-B, blue), NALM-6 (pre-B, orange) and Ramos (B cell, open) stained with HSL11 PE
CD179b, also known as Ig Lambda 5, λ5, and Ig omega, is a 22 kD, C-like protein member within the Ig superfamily. λ5 associates with CD179a (VpreB) to form a surrogate light (SL) chain that in turn associates with the IgM heavy chain and the CD79a/CD79b heterodimer to form the pre-B cell receptor (PreBCR) complex. The function of CD179a/CD179b is still under investigation, though it has been reported that the deficiency of CD179a and/or CD179b impairs the maturation of the B cell precursors beyond the pre-B cell stage.
Other Names:
Ig Lambda 5, λ5, Ig omega
Structure:
Immunoglobine (Ig) V-like protein. Molecular weight 22 kD. Associates with CD179a (VpreB) to form the surrogate light (SL) chain, which binds to the the Ig µ heavy chain and CD79a/CD79b heterodimer complex to form the pre-B cell receptor (preBCR).
Distribution:
Pro-B and early Pre-B cells
Antigen References:
1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc. 2. Zhang M, et al. 2004. Cell. Mol. Immunol. 1:89. 3. Hendriks RW and Middendorp S. 2004. Trends Immunol. 25:249.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD179b (Ig λ5)
Human cell lines REH (pro-B, blue), NALM-6 (pre-B, orange) and Ramos (B cell, open) stained with purified HSL11 conjugated with PE
PE anti-human CD179b (Ig λ5)
Human cell lines REH (pro-B, blue), NALM-6 (pre-B, orange) and Ramos (B cell, open) stained with HSL11 PE
