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The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
The 1A5 antibody cross-reacts with Siglec-144. Additional reported applications (for the relevant formats) include: ELISA2.
Application References:
1. Cornish AL, et al. 1998. Blood 92:2123. 2. Nguyen DH, et al. 2006. P. Natl. Acad. Sci. USA 103:7765. (FC ELISA) 3. Connolly NP, et al. 2002. Br. J. Haematol. 119:221. 4. Angata T, et al. 2006. FASEB J. 20:1964. 5. Avril T, et al. 2005. J. Biol. Chem. 280:19843.
Human peripheral blood granulocytes were stained with Siglec-5 (clone 1A5) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
CD170, also known as Siglec-5, is a single pass transmembrane protein member of the immunoglobulin superfamily. On the cell surface, CD170 forms a 140 kD dimer. The cytoplasmic domain of Siglec-5 contains 2 ITIM motifs that recruit the tyrosine-phosphatases SHP-1 and SHP-2 after tyrosine-phosphorylation, which in turn results in the inhibition of cell signaling. Siglec-5 is expressed by granulocytes, monocytes/macrophages, subsets of lymphocytes, and a subset of activated dendritic cells. Siglec-5 binds α2,3- and α2,6-linked sialic acid as well as glycophorin A, and is involved in cell adhesion.
Other Names:
OBBP2, CD33L2, OB-BP2
Structure:
Member of the immunoglobulin superfamily, single pass transmembrane protein, forms a 140 kD dimer; cytoplasmic domain contains 2 ITIM motifs
Distribution:
Granulocytes, monocytes/macrophages, subsets of lymphocytes, and activated dendritic cells
Function:
Inhibitory receptor, cell adhesion
Ligand Receptor:
α2,3- and α2,6-linked sialic acid, glycophorin A
Interaction:
SHP-1, SHP-2
Antigen References:
1. Soto PC, et al. 2010. J. Immunol. 184:4185. 2. Carlin AF, et al. 2009. J. Exp. Med. 206:1691. 3. Zhuravleva MA. 2008. J. Mol. Biol. 375:437.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD170 (Siglec-5)
Human peripheral blood granulocytes were stained with Siglec-5 (clone 1A5) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
PE anti-human CD170 (Siglec-5)
Human peripheral blood granulocytes were stained with Siglec-5 (clone 1A5) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
