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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Clone GHI/61 binds to domain 7 of CD163. Additional reported applications (for the relevant formats) include: immunoprecipitation1.
Application References:
1. Pulford K, et al. 1992. Immunology 75:588. (IP) 2. Law SK, et al. 1993. Eur. J. Immunol. 23:2320. 3. Madsen M, et al. 2004. J. Biol. Chem. 279:51561. 4. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
Human peripheral blood monocytes stained with GHI/61 PE
CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, Hemoglobin-Haptoglobin Complex Receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kuffer cells, monocytes, subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP) resulting in soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammation diseases.
Monocytes, macrophages, Kuffer cells, subset of dendritic cells, subset of hematopoietic stem/progenitor cells
Function:
Clearance of haptoglobin-hemoglobin complex, regulation of cytokine production by macrophages
Ligand Receptor:
Haptoglobin-hemoglobin complex, TWEAK
Antigen References:
1. Roth J, et al. 1994 Transolantation. 57:127 2. Van den Heuvel MM, et al.1999 J. Leukoc. Biol. 66:858 3. Sulahian TH, et al. 2000 Cytokines 12:1312 4. Fabriek BO, et al. 2007 J. Neuroimmunol. 187:179
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD163
Human peripheral blood monocytes stained with purified GHI/61, followed by anti-mouse IgG FITC
Biotin anti-human CD163
Human peripheral blood monocytes stained with biotinylated GHI/61, followed by Sav-PE
PE anti-human CD163
Human peripheral blood monocytes stained with GHI/61 PE
PerCP/Cy5.5 anti-human CD163
Human peripheral blood lymphocytes, monocytes, granulocytes stained with GHI/61 PerCP/Cy5.5
APC anti-human CD163
Human peripheral blood monocytes stained with GHI/61 APC
